雌激素对PDGF诱导的血管平滑肌细胞增殖及细胞周期的影响  

Effect of estrogen on proliferation and cell cycle of cultured rabbit vascular smooth muscle cells induced by platelet-derived growth factor

作　　者：周永兰[1] 陈清枝[2] 张延斌[2] 王人彭[1] 张义勤[1] 骆秉辁[1] 

机构地区：[1]江苏大学附属医院徐州第三人民医院心内科,江苏徐州221005 [2]徐州医学院附属医院心内科,江苏徐州221002

出　　处：《心脏杂志》2006年第6期611-613,共3页Chinese Heart Journal

摘　　要：目的探讨17β-雌二醇(E2)对血管平滑肌细胞(VSMC)增殖及细胞周期的影响。方法分离培养VSMC,血小板源生长因子(PDGF)诱导其增殖,应用MTT法及流式细胞仪检测不同浓度E2(10和100 nmol/L)对传代VSMC增殖活性及细胞周期的影响。结果E2(10和100 nmol/L)作用下VSMC增殖活性下降,且与浓度有关;对VSMC细胞周期的分布影响主要表现为处于G0/G1期细胞数增多,G2/S期的细胞数减少(P<0.01)。结论E2具有抑制VSMC增殖的作用,其部分机制可能是通过阻滞VSMC G0/G1期向S的转化有关。AIM To investigate the effect of 17β estradiol （ E2 ） on the proliferation of vascular smooth muscle cells （VSMC） and the possible underlying mechanisms. METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGF-BB was established. The proliferation was determined with MTT metabolism in subcuhured VSMC exposed to different concentrations （ 10 and 100 nmol/L） of 1713 estradiol and the cell cycle was examined by flow cytometry. RESULTS MTT metabolism of VSMCs induced by PDGF-BB was inhibited by E2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly （P 〈 0.01 ）. CONCLUSION 1713 estradiol inhibits the proliferation of VSMC induced by PDGF-BB, at least in part by preventing VSMCs entering S phase from G0/G1 phase.

关 键 词：雌二醇 血管平滑肌细胞 增殖 细胞周期 血小板源生长因子 

分 类 号：R458.7[医药卫生—治疗学]