白藜芦醇对外周血内皮祖细胞功能的影响  

Effects of resveratrol on function of endothelial progenitor cells from peripheral blood

作　　者：顾俊[1] 王长谦[1] 范华骅[2] 聂晓绚[2] 王彬尧[1] 黄定九[1] 

机构地区：[1]上海交通大学医学院附属仁济医院心内科,上海200001 [2]上海血液中心血液工程研究室,上海200051

出　　处：《心脏杂志》2006年第6期617-621,共5页Chinese Heart Journal

基　　金：国家自然科学基金资助项目(No.30170365)

摘　　要：目的观察不同浓度白藜芦醇对于内皮祖细胞体外功能的影响。方法密度梯度离心法获得人外周血单个核细胞,培养7 d后,采用免疫荧光和流式细胞仪进行细胞鉴定;随后加入不同浓度的白藜芦醇[0(对照)、1、5、15、60μmol/L]干预72 h,观察其对内皮祖细胞增殖、迁移和黏附能力的影响;同时采用RT-PCR和ELISA方法测定药物干预后,内皮祖细胞中内皮型一氧化氮合成酶(endothelial n itric oxide synthase,eNOS)的表达。结果与对照组相比,1μmol/L白藜芦醇促进内皮祖细胞增殖、迁移和黏附,同时上调eNOS的mRNA和蛋白水平的表达;而60μmol/L白藜芦醇抑制内皮祖细胞增殖、迁移和黏附,并且下调eNOS的mRNA和蛋白水平的表达。结论低浓度白藜芦醇可改善内皮祖细胞增殖、迁移和黏附功能,并上调eNOS表达,而高浓度白藜芦醇则抑制上述细胞功能和eNOS表达。AIM To investigate the effect of resveratrol on the function of endothelial progenitor cells （EPCs）. METHODS Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and cultured. After 7 days of culture in vitro, EPCs were identified as adherent cells double positive for DiI-acLDL/FITC-UEA- I under a laser scanning confocal microscope and their expression of CD34, CD31 and KDR was detected by flow cytometry. EPCs were then stimulated with resveratrol （ 1, 5, 15, 60 μLmol/L） or vehicle control for 72 hours. EPCs＇ proliferation, migration and adhesion activity were assayed. RT-PCR and ELISA were also performed to determine the eNOS expression after resveratrol interference. RESULTS Incubation of human EPCs with low concentration of resveratrol increased the proliferation, adhesion and migration activity. In addition, low concentration upregulated the eNOS expression. While high concentration of resveratrol inhibited aforementioned functions and eNOS expression. CONCLUSION Low concentration of resveratrol may promote the functional activity and eNOS expression of EPCs, but high concentration has adverse effects.

关 键 词：白藜芦醇 内皮祖细胞 内皮型一氧化氮合成酶 

分 类 号：R453[医药卫生—治疗学] R329.2[医药卫生—临床医学]