脑梗死患者外周血淋巴细胞神经营养因子及受体mRNA和相关蛋白质产物的表达  

Expressions of neurotrophic factors,receptors mRNA and related proteins of peripheral blood lymphocyte in cerebral infarction patients

作　　者：方琪[1] 董万利[1] 徐耑[1] 张正春[1] 袁霞[1] 蒋建华[1] 倪健强[1] 

机构地区：[1]苏州大学附属第一医院神经内科,215006

出　　处：《江苏医药》2006年第12期1101-1102,共2页Jiangsu Medical Journal

基　　金：江苏省科技厅社会发展计划资助(BS2003014)

摘　　要：目的研究脑梗死后外周血淋巴细胞神经营养因子(NGF、BDNF、NT-3)及受体mRNA和相关蛋白质表达变化。方法用RT-PCR和Westernblot技术观测外周血淋巴细胞神经营养因子及受体mRNA和相关蛋白质的动态变化。结果急性期外周血淋巴细胞NT-3及受体mRNA和相关蛋白质表达较对照组明显减少(P<0·01),恢复期逐渐上升;急性期NGF与BDNF及其受体mRNA和相关蛋白质表达较对照组显著增加(P<0·01),恢复期无明显表达。结论脑梗死急性期时NGF与BDNF及其受体mRNA和相关蛋白质表达增加,而NT-3及受体mRNA和相关蛋白质表达在缺血初期下降,提示缺血早期NT-3可增加神经元死亡。Objective To study the changes of brain neurotrophic factors in the patients with cerebral infarction. Methods The neurotrophic factors,receptors mRNA and related protein expressions of peripheral blood lymphocyte were estimated with RT-PCR and Western blot. Results At acute stage, the levels of neurotrophic factors, receptors mRNA and related protein expressions of peripheral blood lymphocyte in patients with cerebral infarction were significantly higher than those in control group（P〈0.01）. But there were no significantly differences at convalescence stage. Conclusion At acute stage of cerebral infarction, the levels of NGF and BDNF expressions increased,while NT-3 decreased, indicating that NT-3 may promote neuron death.

关 键 词：神经营养因子 脑梗死 外周血淋巴细胞 受体 

分 类 号：R593.241[医药卫生—内科学]