一氧化氮合酶抑制剂对宫内窘迫胎鼠脑神经细胞凋亡和幼年学习记忆能力的影响  

The effect of NOS inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood's learning and memory

作　　者：张华[1] 吴虹霆[1] 张建华[1] 姚珍薇[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2006年第6期857-860,共4页Journal of Chongqing Medical University

摘　　要：目的:研究一氧化氮合酶抑制剂对宫内窘迫胎鼠脑神经细胞凋亡和幼年学习记忆能力的影响及其抑制剂的保护作用。方法:①建立胎鼠宫内窘迫模型。②分为对照组、缺血再灌注组、治疗组[缺血前1.5h先注射左旋硝基精氨酸(L-NNA),然后于术前即刻腹腔内注射氨基胍(AG)500m g/kg]、远期观察Ⅰ、Ⅱ、Ⅲ组(将对照组、缺血再灌注组和治疗组中的部分胎鼠继续妊娠至孕19天,以剖宫产娩出,饲养至40天)。③检测脑组织中细胞色素C的胞浆释放量,以M orris水迷宫检测幼鼠学习能力。结果:①缺血再灌注组和治疗组胞浆细胞色素C含量均高于对照组,治疗组各时间点的胞浆细胞色素C含量低于缺血再灌注组(P<0.05)。②各组幼鼠历时5天训练,共8个时间段。远期观察Ⅱ组8次平均潜伏期与Ⅰ、Ⅲ组比较,明显延长(P<0.05)。远期观察Ⅲ组与Ⅰ组比较,潜伏期虽然延长,但无统计学意义(P>0.05)。③幼鼠在第5天进行的跨平台次数,远期观察Ⅱ组较Ⅰ组、Ⅲ组明显减少(P<0.05)。而远期观察Ⅲ组与Ⅰ组比较,无显著性差异(P>0.05)。结论:NOS抑制剂通过减轻缺血缺氧脑损伤时胎鼠脑神经细胞凋亡对其出生后的远期智能起保护作用。NOS抑制剂可能作为一种有价值的治疗方法应用于宫内窘迫的治疗中。Objective：Evaluating the effect of nitric oxide synthase （NOS） inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood＇s learning and memory. Methods： ① Set up fetal rat intrauterine distress model. ②Divide into the control group, reperfusion group, treatment group [injected N omega-nitro-L-arginine （L-NNA） 4mg/kg into pregnant rat＇ s abdomen before ischemia and injected aminoguanidine （AG） 500mg/kg before operation],long-term observation group Ⅰ , Ⅱ , Ⅲ （the part of rats in control group, reperfusion group, treatment group were performed cesarean section on pregnant 19 days and bred to 40 day of age after delivery）. ③Measuring the content of cytochrome c in cytosol and examined the ability of learning and memory function. Results ： ①The content of cytochrome c in reperfusion group （6/12/24h） and treatment group （6/12/24h） were higher than those in the control group （P〈0.05）. The content of cytochrome c in treatment group （6/12/24h） were lower than those in reperfusion group （P 〈0.05）. （2） Escape latency in long-term observation group Ⅱ were longer than those in long-term observation group Ⅰ 、 Ⅲ （P 〈0.05）. There were no differences in escape latency between long-term observation group Ⅰ and Ⅲ （P 〉0.05）.（3） Crossing platform times in long-term observation group Ⅱ were fewer than those in long-term observation group Ⅱ , Ⅲ（P 〈0.05） There were no differences in crossing platform times between long-term observation group Ⅰ and m （P 〉0.05 ）. Conclusion ： NOS inhibitors play a obvious protective role in long-term intelligence of fetal rat after delivery through relieved neuron apoptosis during hypoxic ischemia encephalopathy. NOS inbibitors can be a valuable therapy method in intrauterine distress treatment.

关 键 词：宫内窘迫 胎鼠 一氧化氮合酶抑制剂 凋亡 学习能力 

分 类 号：R714.51[医药卫生—妇产科学]