衣原体GPIC噬菌体衣壳蛋白Vp1的克隆、表达和鉴定  

Cloning, expression and identification of chlamydial GPIC capsid Vpl protein

作　　者：刘全忠[1] 姚卫锋[1] 齐蔓莉[1] 李燕[1] 王惠平[1] 田敬群[1] 

机构地区：[1]天津医科大学总医院皮肤性病科,300052

出　　处：《中华皮肤科杂志》2006年第12期714-716,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30671879);天津市自然科学基金资助项目(023612111)

摘　　要：目的获得衣原体GPIC噬菌体衣壳蛋白Vp1基因及其蛋白。方法提取噬菌体ΦCPG1及其核酸,并对Vp1克隆、表达,鉴定。结果所获得的Vp1基因片段经测序,长度为1 661 bp,检索确认其序列与Genebank一致。对重组质粒进行诱导表达,SDS-PAGE和蛋白质印迹均显示获得相对分子质量约62 000的衣原体GPIC噬菌体衣壳蛋白Vp1。结论成功获得了噬菌体衣壳蛋白Vp1,为进一步研究打下基础。Objective To clone, express, and identify chlamydial GPIC capsid Vpl gene and protein. Methods The complete sequence of Vpl gene from φCPG1 phage was amplified. The amplicor was cut by restriction endonuclease, linked to plasmid vector, and transformed into E. coli. The expressed protein of recombinant Vpl was purified and identified. Results The recombinant 1 661 bp gene was sequenced and proved to be φCPG1 Vpl by searching Genebank. A 62 kDa capsid protein was expressed and confirmed by SDS-PAGE and Western blot. Conclusion The recombinant Vpl seems to be a highly conserved and specific marker for chlamydial phage.

关 键 词：衣原体噬菌体 VP1蛋白 重组 表达 

分 类 号：R374[医药卫生—病原生物学]