人乳头瘤病毒11型DNA疫苗协同CD80基因诱导小鼠特异性免疫反应  

Enhancement of human papillomavirus type 11 DNA vaccine-induced specific immune response by co-immunization with CD80 co-stimulatory gene

作　　者：李莉华[1] 黄朝晖[1] 郭子健[1] 刘志辉[1] 任金冬[1] 宋明旭[1] 周希科[1] 王飞[2] 毕志刚[2] 

机构地区：[1]苏州大学附属无锡第四医院中心实验室,无锡市肿瘤防治研究所,无锡214062 [2]南京医科大学附属第一医院皮肤科

出　　处：《中华皮肤科杂志》2006年第12期717-719,共3页Chinese Journal of Dermatology

基　　金：江苏省重点学科基金(135-03);江苏省重点学科开放招标项目(WK200221)

摘　　要：目的探讨人乳头瘤病毒(HPV)11E7、L1-E7 DNA疫苗质粒协同共刺激分子CD80诱导的特异性免疫反应。方法将所构建的pcDNA3.1(+)/HPV11E7、E7-CD80、L1-E7 DNA疫苗质粒分别经肌内注射免疫小鼠,并设L1-E7质粒与CD80质粒共同注射组,PCR和RT-PCR检测质粒DNA的组织分布和目的基因RNA转录,MTT法测脾淋巴细胞增殖活性,ELISA检测脾淋巴细胞培养上清细胞因子的表达水平及血清抗体的水平。结果免疫小鼠后,质粒主要分布在注射部位。DNA疫苗诱导机体产生特异性脾淋巴细胞增殖和分泌IL-2、IFN-γ增加,产生HPV11E7 IgG/L1 IgG抗体;与CD80基因嵌合后,脾淋巴细胞增殖和分泌活性显著增强;与CD80联合免疫组所诱导的细胞免疫反应和体液免疫均显著强于L1-E7单独免疫组。结论HPV11 DNA疫苗能诱导小鼠产生特异性免疫反应,CD80可加强其免疫效果。Objective To investigate the specific immune response induced by DNA vaccinal plasmid of human papillomavirus type 11 （ HPV11 ）E7, L1-E7 and enhancement of immune reaction by CD80 co-stimulatory gene. Methods The constructed pcDNA3.1 （＋）/HPV11E7, E7-CD80 and L1-E7 DNA vaccinal plasmid, or combination of CD80 and L1-E7 plasmids, were injected intramuscularly into mice, respectively. The distribution of plasmid DNA and transcription of RNA in tissue were analyzed by PCR and RT-PCR, respectively. The lymphocyte proliferation activity was detected by MTT assay. The level of cytokines was determined by ELISA in supernatants of cultured spleen cells and antibodies in serum. Results After immunization, the recombinant plasmid was mainly located in the injection site. Specific anti-HPV 11 E7 IgG/L1 IgG antibodies, lymphocyte proliferation and secretions of cytokines IL-2 and IFN-γ/were induced by injection of DNA vaccine, The levels of lymphocyte proliferation and cytokine secretion were significantly higher in groups of HPV11E7 in recombinant with CD80 co-stimulatory gene than those without CD80. The levels of specific cellular and humoral immunity were also significantly increased in group with the combination of L1-E7 plasmid and CD 80 than those without CD80. Conclusions HPV11DNA vaccination could induce specific cellular and humoral immunity. CD80 co-stimulatory gene may represent an attractive adjuvant for enhancement of the specific immune responses.

关 键 词：乳头状瘤病毒 人 抗原 CD80 疫苗 DNA 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]