PPARα激动剂抑制脂多糖诱导THP-1细胞的细胞组织因子表达  

PPARα Agonist—Fenofibrate Inhibits LPS-induced Tissue Factor Expression in THP-1 Cells

作　　者：董春霞[1] 胡豫[1] 王华芳[1] 孙春艳[1] 王雅丹[1] 何文娟[1] 张小平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,血液病研究所,武汉430022

出　　处：《中国实验血液学杂志》2006年第6期1227-1230,共4页Journal of Experimental Hematology

摘　　要：本研究探讨过氧化物酶体增殖物激活型受体α(peroxisomeproliferator-activatedreceptor-α,PPARα)的激动剂对单核细胞株THP-1中组织因子(tissuefactor,TF)表达的影响。用不同浓度的PPARα激动剂非诺贝特(fenofibrate)预处理单核细胞THP-1一定时间,然后分别用RT-PCR和Westernblot法检测由细菌脂多糖(lipopolysaccharide,LPS)诱导的单核细胞TFmRNA和蛋白质表达水平。结果显示非诺贝特减少了LPS诱导的人单核细胞TFmRNA和蛋白质的表达,并呈剂量依赖性(P<0.05-0.01)。结论非诺贝特抑制了THP-1中LPS诱导的TF表达,此机制可能参与了PPARα激动剂的抗动脉粥样硬化作用。This study was aimed to investigate the influence of PPARα agonist on the expression of TF （ tissue factor） in THP-1 cells. THP-1 cells were pretreated with different concentrations of PPARα agonist（fenofibrate） for definite time. Lipopolysaccharide（LPS）-induced TF mRNA and protein levels were detected by RT-PCR and Western blot respectively. The results showed that fenofibrate decreased tissue factor protein and mRNA expression in supernatants of LPS-stimulated human monocytes in a concentration-dependent manner （ P 〈 0, 05 - 0.01, n = 5 ）. It is concluded that fenofibrate inhibite TF expression induced by LPS in THP-1 cells, which may be involved in the anti-atherosclerotic effects of PPARα agonist.

关 键 词：组织因子 PPARΑ激动剂 非诺贝特 动脉粥样硬化 血栓形成 

分 类 号：R543.3[医药卫生—心血管疾病] R364.15[医药卫生—内科学]