苦叶七种质资源的随机扩增多态性DNA分析  

Random Amplified Analysis of Fagopyrum tataricum from Different Germplasm Resources

作　　者：樊冬丽[1] 牛西午[2] 马小军[1] 肖小河[3] 鄢丹[3] 廖庆文[3] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]山西省农业科学院,山西太原030006 [3]中国人民解放军第302医院中医药研究所,北京100039

出　　处：《时珍国医国药》2006年第12期2471-2473,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨不同苦叶七资源在分子水平上的遗传多样性。方法采用随机扩增多态性DNA(RAPD)技术对30个苦叶七地方品种进行检测,并用NTSYS软件对扩增结果进行统计和聚类。结果80个随机引物中有10个引物的扩增产物具有多态性,每个多态性引物平均可扩增出6个片段。聚类分析表明,全部材料可划分为五个类群。结论苦叶七种质资源在分子水平上存在明显的遗传差异。Objective To explore the genetic diversity of Fagopyrum tataricunt on molecular level. Methods Random amplified polymorphic DNA （ RAPD ）was used to test 30 Fagopyrum tataricum. The NTSYS software was used to analyze the marks. Results Among 80 arbitrary primers, 10 were selected to amplify polymorphic produces,6 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method, all the tested accessions could be clustered into five groups. Conclusion There actually existed much genetic diversity on molecular level among Fagopyrum tataricum germplasm resources.

关 键 词：苦叶七 随机扩增多态性DNA 遗传多样性 

分 类 号：R284.1[医药卫生—中药学]