紫外分光光度法测定芩栀胶囊中黄芩总黄酮和栀子总环烯醚萜苷的含量  

Determination of Total Flavonoid Compounds of Scutellaria baicalensis and Total Glycosides of Gardenia iridoid in Qinzhi Capsule by UV

作　　者：姚干[1] 何宗玉[1] 

机构地区：[1]重庆邮电大学生物信息学院,重庆400065

出　　处：《时珍国医国药》2006年第12期2474-2475,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立芩栀胶囊中黄芩总黄酮和栀子总环烯醚萜苷的含量测定方法。方法采用紫外分光光度法进行测定。样品与三氯化铝反应，形成络合物，检测黄芩总黄酮含量，波长290nm。采用背景消除法，检测栀子总环烯醚萜苷含量，波长：238nm。方法采用紫外分光光度法进行测定。样品与三氯化铝反应，形成络合物，检测黄芩总黄酮含量，波长290nm。采用背景消除法，检测栀子总环烯醚萜苷含量，波长：238nm。结果黄芩总黄酮在5．28～10.56μg/ml范围内线性关系良好，r=0.999997，平均加样回收率为98.33％，RSD=1．05％。栀子总环烯醚萜苷在19．96～31．94μg／ml范围内线性关系良好，r=0．999806，平均加样回收率为98．80％，RSD=0，94％。结论该方法简便、快速、准确．可用于苓栀胶囊的质量控制。结论该方法简便、快速、准确，可用于芩栀胶囊的质量控制。Objective To establish the determination method for total flavonoid compounds Scutellaria baicalensis and total glycosides Gardenia iridoid in Qinzhi capsule. Methods After the sample was reacted with aluminum trichloride, the total flavonoid compounds ScuteUaria baicalensis was determined with the detection wavelength 290nm by UV. After the background interferer in the sample was eliminated ,the total glycosides Gardenia iridoid was determined with the detection wavelength 238nm by UV. Results For total flavonoid compounds Scutellaria baicalensis, there was good linear relationship in the range of 5.28～0.56μg/ ml, r =0. 999 997 with the average recovery 98, 33% and RSD 1.05%. For total Gardenia iridoid glycosides, there was good linear relationship in the range of 19.96～31.94μg/ml, r =0.999 806 with the average recovery 98.80% and RSD 0. 94%. Conclusion This method is simple, quick, accurate and with good reproducibility. It can be used for the quality control of Qinzhi capsule.

关 键 词：芩栀胶囊 黄芩总黄酮 栀子总环烯醚萜苷 紫外光谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]