根癌农杆菌介导AtNHX1基因转化紫花苜蓿的研究  

Transformation studies of Medicago saliva mediated by Agrobacterium tumefaciens with AtNHX1 gene

作　　者：王强龙[1] 王锁民[1] 张金林[1] 包爱科[1] 陈托兄[1] 楼洁琼[1] 陆妮[1] 

机构地区：[1]兰州大学草地农业科技学院

出　　处：《草业科学》2006年第12期55-59,共5页Pratacultural Science

基　　金：国家自然科学基金项目(30671488);新世纪优秀人才支持计划(NCET-05-0882)

摘　　要：在已建立的紫花苜蓿Medicogo sativa体细胞胚高频再生体系的基础上,研究了OD600、侵染时间、负压处理、预培养及共培养时间、脱菌及选择方法等因子对转化效率的影响,并初步获得了抗性体胚。Several factors （bacterial concentrations, infected time, negative pressure treatment time, pre-culture and co-culture time, acterium free and screening methods etc. ） affecting genetic transfor mation of alfalfa （Medicago sativa）, which is mediated by Agrobacterium tumefaciens with AtNHX1 gene, were studied on the basis of high-frequency regeneration system of alfalfa somatic embryos. Transformation system for alfalfa was successfully set up and the transgenic somatic embryos were also obtained.

关 键 词：紫花苜蓿 体细胞胚 根癌农杆菌 ATNHX1 遗传转化 

分 类 号：S541.9[农业科学—作物学]