超临界CO_2流体萃取与乙酸乙酯提取蛇床子化学成分的对比  

Comparison of the Extracts from Fructus Cnidii Prepared by Supercritical Fluid Extraction and by Ethyl Acetate Extraction

作　　者：张晓霞[1] 周卯星[1] 张高勇[1] 关景才[1] 宫竹云[1] 

机构地区：[1]中国日用化学工业研究院表面活性剂国家工程研究中心,山西太原030001

出　　处：《精细化工》2006年第12期1216-1220,共5页Fine Chemicals

基　　金：山西省高级专家项目(204512)~~

摘　　要：用超临界CO2流体萃取（SFE—CO2）和乙酸乙酯提取（SE）蛇床子中的有效成分，采用气相色谱一质谱法（GC—MS）对SFE—CO2和SE提取物进行了化学成分的对比研究。鉴定出SE提取物的43个成分，SFE—CO2提取物的39个成分，占各提取物质量的80％-90％；sE提取蛇床子有效成分的能力较弱，而采用SFE—CO2萃取釜压力40MPa，温度40℃，解析釜Ⅰ、Ⅱ压力5MPa，解析釜Ⅰ温度45oC，解析釜Ⅱ温度46℃的工艺条件萃取出的蛇床子提取物，较SE选择性强，有利于萃取总香豆素以及萜烯类化合物；萃取总香豆素的质量分数（35.32％）比SE（17．39％）提高了18％，尤其萃取蛇床子素（23．83％），约为SE（6．32％）的4倍；萜烯类化合物（5．35％）也比SE（2．09％）提高了3％。The extracts from fructus Cnidii were obtained by different methods including supercritical carbon dioxide fluid extraction （ SFE - CO2 ） and ethyl acetate solvent extraction （SE）. The chemical constituents of these different extracts were compaired by studying with GC - MS. Forty-three chemical constituents by SE and thirty-nine by SFE -CO2 were identified, contributing to about 80% -90% of total peak areas of the extracts fromfructus Cnidii. Under the extraction conditions that extraction kettle pressure was 40 MPa, extraction kettle temperature was 40℃, resolver Ⅰ and Ⅱ pressure was 5 MPa, resolver Ⅰ temperature was 45 ℃, and resolver Ⅱ temperature was 46 ℃the selectivity of SFE - CO2 was better than that of SE, suitable for total coumarin and terpene. Comparied with SE, the mass fraction of total coumarin by SFE - CO2 （35.32%） increased by 18%. Especially the mass fraction of ostholeby SFE- CO2（23.83% ） was 4 times that by SE（6. 32% ） ,and the mass fraction of terpene by SFE - CO2 increased by 3%.

关 键 词：蛇床子提取物 超临界CO2 流体萃取 溶剂提取 

分 类 号：TQ420.66[化学工程]