p38 MAPK信号转导通路参与NO诱导兔关节软骨细胞凋亡  

The p38 MAPK signal transduction pathway is involved in rabbit articular chondrocyte apoptosis induced by NO

作　　者：王红林[1] 王治伦[1] 吴劲[1] 考希宾[1] 高艳[1] 

机构地区：[1]西安交通大学医学院地方病研究所,西安710061

出　　处：《中国地方病防治》2006年第6期332-335,351,共5页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金资助项目(30371245和39770667)

摘　　要：目的探讨p38 MAPK信号转导通路在软骨细胞凋亡中的作用。方法体外培养兔关节软骨细胞,一氧化氮(NO)供体NOC-18和p38 MAPK抑制剂SB203580作用于细胞24 h,用AnnexinV-FITC/PI流式细胞术检测软骨细胞凋亡率,W estern b lot测定p38、磷酸化p38蛋白的表达水平。结果与对照组比较,SB203580显著降低了NOC-18诱导的软骨细胞凋亡率(P<0.05);NOC-18以浓度依赖的方式促进p38 MAPK的磷酸化,而SB203580能抑制其磷酸化(P<0.05)。结论p38 MAPK通路参与了NO诱导的兔关节软骨细胞凋亡的信号转导。Objective To explore the role of p35 MAPK signal transduction pathway in chondrocyte apoptosis. Methods Rabbit articular chondrocytes were cultured in vitro. Following treatment with NO donor NOC - 18 and p38 MAPK inhibitor SB203580 for 24 h, we performed Annexin V - FITC/PI flow cytometry （FCM） to detect chondrocyte apoptosis rate, and Western blotting to determine the protein expression of p38, phosphorylation p38. Results SB203580 significantly decreased chondrocyte apoptosis rate, compared with untreated control （ P 〈0. 05） ; NOC -18 promoted the phosphorylation of p38 MAPK in a concentration - dependent manner, and SB203550 could inhibit it （ P 〈 0. 05）. Conclusions The p38 MAPK pathway is involved in the signal transduction of NO - induced rabbit articular chondrocyte apoptosis.

关 键 词：NO 软骨细胞 凋亡 P38 

分 类 号：R977.4[医药卫生—药品]