VEGF抗体及孕酮对无排卵性功能失调性子宫出血患者子宫内膜间质细胞MMP-9表达的影响  

Adjustment of matrix metalloproteinase-9 in endometrial stromal cells of dysfunctional uterine bleeding by VEGF antibody and progesterone

作　　者：孙燕[1] 乔福元[1] 岳锦春[2] 徐金霞[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030 [2]广西医科大学附属第一医院妇产科,南宁530027

出　　处：《中国优生与遗传杂志》2006年第12期20-21,34,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨孕激素、细胞因子和基质金属蛋白酶-9在更年期无排卵性子宫出血发病机制中的作用及相互关系。方法采集对照组和功血组患者子宫内膜各11例和16例,原代培养子宫内膜间质细胞。在对照组和功血组内设置空白对照组、孕酮组和VEGF抗体组,孕酮组分别加入孕酮10-7mol/L(生理浓度),10-5mol/L(高浓度),各孵育24h。VEGF抗体组加入VEGF抗体10μl,分别孵育24h,48h,ELISA法检测MMP-9含量。结果功血组患者子宫内膜间质细胞MMP-9含量明显高于对照组,加入孕酮作用后,各组MMP-9含量均下降,功血组下降更明显,高浓度孕酮有更强的抑制作用(P<0.05)。VEGF抗体作用后各组MMP-9含量均下降,以功血组明显(P<0.05)。不同作用时间对MMP-9含量无显著影响,(P>0.05)。结论功血患者子宫内膜间质细胞分泌较MMP-9较对照组高,VEGF抗体和孕酮均能下调MMP-9水平,提示VEGF和MMPs均参与了功能失调性子宫出血的病理过程。Objective： To evaluate the roles of cytokine, sex hormone and matrix metalloproteinase -9 in endometrial stromal cells of dysfunctional uterine bleeding by VEGF antibody and progesterone. Methods： Sixteen patients with DUB were included as study group and eleven patients without DUB as control group. Concentrations of MMP -9 were determined by ELISA. Results： Levels of MMP -9 in the DUB group was significantly higher than the control group, Secretion of MMP -9 in each group was decreased in culture with progesterone and VEGF antibody, levels of MMP - 9 in both groups were significantly decreased in the higher concentration of progesterone. Conclusion： VEGF and MMPs may play a role in pathogenesis of DUB, Progesterone can decrease the secretion of MMP -9 and it may restrain the development of DUB.

关 键 词：功能失调性子宫出血 基质金属蛋白酶 细胞因子 孕酮 

分 类 号：R711.52[医药卫生—妇产科学]