孕妇生殖道支原体感染的实验室诊断及方法学评价  

Methodology valuation and laboratory diagnosis on mycoplasma of genital tract of pregnant women

作　　者：李荣国[1] 高亚丽[1] 孙恩华[2] 芦云娥[1] 张霞[1] 

机构地区：[1]山东济南市妇幼保健院检验科,济南250001 [2]山东大学齐鲁医院检验科,济南250012

出　　处：《中国优生与遗传杂志》2006年第12期105-106,97,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨孕妇生殖道支原体感染的实验室诊断及方法学评价,以寻找一种适合孕期常规查体的方法。方法2004年1月至2004年6月,检验科随即机对200名孕妇的阴道分泌物同时运用超高倍显微镜、支原体(Uu、Mh)分离鉴定试剂盒及聚合酶链反应(PCR)技术进行支原体检测。结果方法一:超高倍显微镜检测阳性率29.5%(59/200)。方法二:支原体(Uu、Mh)分离鉴定试剂检测阳性率25%(50/200)。方法三:PCR检测阳性率18.5%(37/200)。应用SPSS软件包分析:方法一与方法二无显著差异(P>0.05),方法一与方法三有显著差异(P<0.05)。结论超高倍显微镜在孕妇生殖道的支原体活体检测方面有一定的诊断价值,其准确性较PCR技术有一定的差距,建议用于病原微生物的初筛诊断试验,确诊应与临床表现和其他确诊试验相结合。Objective： To search a method suitable for routine examination by investigating the methodology valuation and laboratory diagnosis on mycoplasma infection of genital tract of pregnant women. Methods ： Clinical laboratory had examined the mycoplasma in the vaginal discharge of 200 pregnant women in our hospital from Jan. 2004 - June 2004 by the multimedia microscopy, mycoplasma reagent identification and PCR, Results： The first method： the positive rate of examination by the multimedia microscopy is 29, 5% （59/200）. The second method： the positive rate of examination by mycoplasma reagent identification is 25% （50/200）. The third method： the positive rate of examination by PCR is 18.5% （37/200）. Analyzed by SPSS software, there was not statistical difference between the first and the second method （ P 〉 0, 05 ） , there was statistical difference between the first and the third method, between the first and the third method （ P 〈 0. 05）, Conclusion： It has a certainty diagnosis valuation that the application of multimedia microscopy in examining mycoplasma of genital tract of pregnant women, but the veracity is lower than the technique of PCR. It is adviced to used with a first examination.

关 键 词：孕妇 生殖道 支原体 超高倍显微镜 PCR 方法学 

分 类 号：R714.2[医药卫生—妇产科学]