旋毛虫幼虫的组织化学观察  

Histochemical Observations on the Trichinella spiralis Larva

作　　者：杨元清[1] 张超威[1] 吴嘉彤[1] 常正山[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《地方病通报》1996年第4期7-9,共3页Endemic Diseases Bulletin

摘　　要：本文对感染河南株旋毛虫６１０±１０条后７．５个月的昆明品系小白鼠膈肌内幼虫的组织化学进行了较为全面的观察。结果显示旋毛虫幼虫含有糖原、ＤＮＡ、ＲＮＡ、蛋白质结合的α－氨基、酪氦酸、色氨酸及组氨酸、碱性蛋白质、脂肪、酸性粘多糖、淀粉样物质、玻璃样物质、胶原及网状纤维、ＡＫＰ、ＡＣＰ及ＡＴＰ酶等物质，对上述物质的定位、含量或活力作了较为详细的描述，并对其生理意义进行了讨论。Biochemical substance of Trichtnella spiralis larva in mouse diaphragmatic muscle were inves-tigated by histochen1ical techniques.Five Kunming strain mice were inoculated with 610±10 Henan strain larvae of T.spiralis per mouse andwere killed in 7.5 months after infection,the diaphragmatic muscle was taken for histochemical exaininations.The results showed that the T.spiralis larva contained glycogen,DNA,RNA,protein bound α-aminogroups,protein bound tyrosine,tryptophan and histidine,alkaline protein,lipid,acid mucopolysaccharide,amy-loid substance,hyaline substance,collagenous and reticular fiber elements,AKP,ACP and ATPase.The loca-tion,content and activity of above biochemical substances were described as the follows:A heavy deposits of glycogen were found in muscular layer of muscle larva,moderate content or a little inthe cytoplasm of the other cells. Both the DNA and RNA were mainly located in nucleus,but very rich in gen-ital primordium.As to the protein bound amino acid groups and protein of muscle larva,which were chieflydistributed in nucleus,genital primordium and muscular layer,but richer content was observed in genital pri-mordium.The collagenous and reticular fiber element were mainly situated in cuticle and subcuticle of larva,and both had very rich content respectively. Activities of AKP,ACP and ATPase of muscle larva were almostmainly limited in cuticle and genital primordium,but strongger activity was seen in cuticle than in genital pri-mordium. The content of lipid,acid mucopolysaccharide,amyloid substance and hyaline substance was almostinvestigated a little in muscular larva of T.spiralis.The physiological significance of histochemical elements in muscle larva of T.spiralis mentioned abovewas discussed briefly.

关 键 词：旋毛虫 河南株 膈肌 成囊期幼虫 组织化学 

分 类 号：R383.15[医药卫生—医学寄生虫学]