老年性痴呆模型小鼠脑组织中FKBP-12表达水平研究  

Study of the expression level of FKBP-12 in Alzheimer's disease model mice's brains.

作　　者：何有娣[1] 黎燕[2] 陈天风[1] 胡文立[1] 舒翠玲[2] 

机构地区：[1]首都医科大学北京朝阳医院神经内科,北京100020 [2]军事医学科学院,北京100850

出　　处：《中国实用内科杂志：临床前沿版》2006年第12期1941-1943,共3页

基　　金：国家"973"创新药物基金(G1998051107)

摘　　要：目的研究他克莫司结合蛋白-12(FKBP-12)在老年性痴呆模型小鼠及正常小鼠脑内表达量的差异。方法2001年2月至2002年5月,首都医科大学北京朝阳医院神经内科选用日本自然快速老化的小鼠SAM-P/8为老年性痴呆模型,以小鼠SAM-R/1为正常对照,用逆转录聚合酶链反应(RT-PCR)和免疫组化染色的方法,从mRNA水平和蛋白水平,在4、6、8、10个月4个不同的月龄组中,观察FKBP-12在老年性痴呆模型小鼠体内及正常小鼠体内表达量。结果在4、6、8、10个月4个不同的月龄组中,在mRNA水平及在蛋白水平,均未检测到FKBP-12在老年性痴呆模型小鼠及正常小鼠脑组织中表达量的差异。结论FKBP-12可能与日本老年性痴呆模型小鼠的早老过程无明显相关性。Objective To observe the difference of FKBP - 12 expression level between in Alzheimer＇s disease model mice＇s brains and in control mice＇s brains. Methods Taking the Senescence Accelerated Mouse imported from Japan （SAM -P/8） as the Alzheimer＇s disease model mouse, and SAM -R/1 mouse as control, observe the distribution difference of FKBP - 12 between in Alzheimer＇s disease model mice＇s brains and in control mice＇s brains both in mRNA level and in protein level with the methods of RT - PCR and immunohistochemistry staining. The mice were divided into four groups of 4, 6, 8, 10 months old. Results There was no distribution difference of FKBP - 12 between in Alzheimer＇s disease model mice＇s brains and in control mice＇s brains both in mRNA level and in protein level. Conclusion FKBP - 12 probably has no relativity with the accelerated aging process of the Alzheimer＇s disease model mice imported from Japan.

关 键 词：他克莫司结合蛋白-12 老年性痴呆模型小鼠 基因表达 

分 类 号：R5[医药卫生—内科学]