端粒酶 血管内皮生长因子 粘附分子 基质金属蛋白酶检测对胸腔积液性质鉴别的临床意义  

Differential diagnosis meaning of telomerase,VEGF,CD44_ V6 ,MMP_2,MMP_9 and their combined test in pleural effusions.

作　　者：孙瑜[1] 王惠军[2] 李翊卫[2] 王良兴[3] 

机构地区：[1]浙江海洋学院医学院,浙江舟山316000 [2]舟山市人民医院,浙江舟山316004 [3]温州医学院,浙江温州325003

出　　处：《中国实用内科杂志：临床前沿版》2006年第12期1968-1970,共3页

基　　金：浙江省教育厅科研计划项目(20050121)

摘　　要：Objective To observe the diagnostic value of telomerase,VEGF,CD44 V6 ,MMP2,MMP9and their combined examination in distinguishing malignant pleural effusion from benign one.Methods Sixty patients with pleural effusions were observed,thirty of them sufferring from malignant pleural effusion and thirty of them sufferring from benign one.The expression of telomerase,CD44 V6 ,VEGF,MMP2,MMP9were tested in the pleural effusion by RT-PCR and ELISA technique.The sensitivity and the specificity of each examination method were calculated,in order to find out appropriate combination.Results As single-item detection,the diagnosetic value of VEGF was the best,the cutoff of VEGF was 181.1pg/ml,the sensitivity of VEGF examination was 90.0%,and the specificity was 86.7%.The sensitivity and the specificity of combined examination VEGF+CD44 V6 and VEGF+MMP2 were the best,the sensitivity of VEGF+CD44 V6 was 83.3%,and the specificity was 90.0%.The sensitivity of VEGF+MMP2 was 80.0% and the specificity was 93.3%.Conclusion The level of telomerase,VEGF,CD44 V6 ,MMP2,MMP9in malignant pleural effusion is clearly higher than that in benign pleural one,the difference having significant statistic meaning.VEGF has higher sensitivity and specificity .Of combined examination,VEGF+CD44 V6 and VEGF+MMP2 have higher sensitivity and specificity.Objective To observe the diagnostic value of telomerase, VEGF, CD44V6, MMP2, MMPgand their combined examination in distinguishing malignant pleural effusion from benign one. Methods Sixty patients with pleural effusions were observed, thirty of them sufferring from malignant pleural effusion and thirty of them sufferring from benign one. The expression of telomerase, CD44V6, VEGF, MMP2, MMPgwere tested in the pleural effusion by RT - PCR and ELISA technique. The sensitivity and the specificity of each examination method were calculated, in order to find out appropriate combination. Results As single - item detection, the diagnosetic value of VEGF was the best, the cutoff of VEGF was 181. 1pg/ml, the sensitivity of VEGF examination was 90. 0%, and the specificity was 86. 7%. The sensitivity and the specificity of combined examination VEGF ＋ CD44v6 and VEGF ＋ MMP2 were the best, the sensitivity of VEGF ＋ CD44v6 was 83.3%, and the specificity was 90. 0%. The sensitivity of VEGF ＋ MMP2 was 80. 0% and the specificity was 93. 3%. Conclusion The level of telomerase, VEGF, CD44v6, MMP2, MMPgin malignant pleural effusion is clearly higher than that in benign pleural one, the difference having significant statistic meaning. VEGF has higher sensitivity and specificity . Of combined examination, VEGF ＋ CD44v6 and VEGF ＋ MMP2 have higher sensitivity and specificity.

关 键 词：胸腔积液 端粒酶 血管内皮生长因子 粘附分子 基质金属蛋白酶 鉴别诊断 联合检测 

分 类 号：R5[医药卫生—内科学]