rhIL-11诱导转录因子GATA-1和NF-E2在Dami细胞的表达  

Recombinant human interleukin 11 up-regulates the expression of transcription factor GATA-1 and NF-E2 in Dami cells.

作　　者：白松婷[1] 盛光耀[1] 卢洁[1] 赵晓明[1] 方营旗[1] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052

出　　处：《山东医药》2006年第34期16-17,共2页Shandong Medical Journal

摘　　要：目的探讨重组人白细胞介素11(rh IL-11)对巨核细胞白血病细胞株D am i内转录因子GATA-1和NF-E 2表达的调节作用。方法用rh IL-11(50 ng/m l)刺激D am i细胞1、2、4 h,W estern b lot和RT-PCR法分析rh IL-11诱导D am i细胞GATA-1、NF-E 2 mRNA表达水平。结果rh IL-11可诱导D am i细胞GATA-1、NF-E 2mRNA表达,1 h后作用下降。结论rh IL-11可能通过诱导GATA-1、NF-E 2表达调控巨核细胞生成。[Objective] To investigate the regulated action of recombinant human interleukin 11（rhIL-11） on transcription factors GATA-1 and nuclear factor erythroid 2 （NF-E2） in human megakaryocytic leukemia cell line Dami. [Methods] RT-PCR and Western blot were used to detecte the expression level of GATA-1 and NF-E2 mRNA in Dami cells induced by rhIL-11（50 ng/ml） for 1 h, 2 h, 4 h. [Results] rhIL-11 was found to increase the expression of GATA-1 and NF-E2 mRNA in Dami cell, but the action was decreased quickely after 1 hour, [Conclusion] rhIL-11 may take part in the regulation of megakaryocyte through increasing the expression of GATA-1 and NF-E2.

关 键 词：巨核细胞 重组人白细胞介素11 转录因子 Dami细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]