二甲基亚硝胺大鼠肝纤维化中医方证研究  

Study on TCM Recipe and Syndrome of Dimethylnitrosamine-induced Hepatic Fibrosis in Rats

作　　者：王磊[1] 刘平[1] 慕永平[1] 李风华[1] 龙爱华[1] 顾宏图[2] 陈高峰[1] 

机构地区：[1]上海中医药大学肝病研究所 [2]上海中医药大学附属曙光医院

出　　处：《中医杂志》2006年第12期929-932,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金重大研究计划重点项目(90409020);上海市自然科学基金重点项目(02JC14024);上海市教育委员会E-研究院建设计划项目(E03008);上海市重点学科建设项目(Y0302)。

摘　　要：目的:探讨二甲基亚硝胺(DMN)大鼠肝纤维化的中医方证。方法:腹腔注射法制备DMN大鼠肝纤维化模型,分别采用5首不同功效的经典方治疗两周,观察各组大鼠肝组织病理学、羟脯氨酸(Hyp)含量及肝功能变化。结果:造模4周后大鼠形成典型小结节性肝硬化,6周后模型组大鼠肝组织损伤较4周时有所缓解。与6周模型组相比,茵陈蒿汤组大鼠肝组织病理变化、Hyp含量及肝功能均显著改善(P<0.05或P<0.01);黄芪汤组大鼠肝组织病理变化、Hyp含量亦显著改善(P<0.05或P<0.01)。结论:茵陈蒿汤、黄芪汤能够有效逆转DMN大鼠肝纤维化,成型期DMN肝纤维化大鼠的主要中医方证为湿热、瘀热内蕴兼气虚。Objective：To probe into TCM recipe and syndrome of dimethylnitrosamine（DMN）-induced hepatic fibrosis of the rat. Methods： DMN rat hapetic fibrosis model was prepared by intraperitoneal injection of DMN,and they were respectively treated with 5 classical recipes with difference functions for 2 weeks. Changes of pathology of the hepatic tissue,hydroxyproline（Hyp） content and hapetie function were investigated. Results： 4 weeks after modeling,typical micronodular cirrhosis formed,and 6 weeks after modeling,the hepatic injury in the model group had some remission as compared with that at 4 weeks. Compared with the model group at 6.weeks,pathologic changes of the hepatic tissue,Hyp content and hepatic function improved significantly （P〈0.05 or P〈0. 01） in the Inchenhao Decoction group;pathologie changes of the hepatic tissue and Hyp content in the Huangqi Decoction group improved significantly （P〈0. 05 or P〈0. 01 ）. Conelusion：Inchenhao Decoction and Huangqi Decoction can effectively reverse DMN-induced hepatic fibrosis of rats;damp-heat,stagnation of heat in the interior with qi-deficiency are main TCM syndromes of DMN hepatic fibrosis rat at the formative stage.

关 键 词：肝纤维化/中医药疗法 

分 类 号：R259[医药卫生—中西医结合]