清肝化瘀方对大鼠肝癌前病变模型Notch信号系统的影响  

Effect of Qinggan Huayu Peroral Solution on Notch Signaling System of Hepatic Precancerous Lesion in Rats

作　　者：杨志云[1] 姚树坤[2] 殷飞[2] 修贺明[3] 

机构地区：[1]河北医科大学 [2]河北医科大学第四医院消化内科 [3]白求恩国际和平医院中心实验室

出　　处：《中医杂志》2006年第12期933-935,共3页Journal of Traditional Chinese Medicine

基　　金：河北省自然科学基金资助项目(C2006000852)

摘　　要：目的:探讨清肝化瘀方对大鼠肝癌前病变模型Notch信号系统的影响。方法:用二乙基亚硝胺(DEN)建立Solt-Farber二步大鼠肝癌前病变的短期实验模型,应用不同浓度的清肝化瘀方进行干预,酶组织化学方法检测三磷酸腺苷酶(ATP酶)的表达变化,免疫组织化学方法检测白蛋白(ALB)、Notch1、Notch1配体Jagged的表达变化。结果:与模型组比较,清肝化瘀方等效剂量、大剂量组ATP酶、ALB表达水平显著升高(P<0.01),Notch1、Jagged表达水平显著降低(P<0.01)。结论:清肝化瘀方可有效阻断大鼠肝癌前病变,其作用机理可能与调节Notch信号通路有关。Objective：To investigate the effect of Qinggan Huayu Peroral Solution on Notch signaling system of hepatic precancerous lesion in rats. Mehtods：A Solt Farber two-step test model of prehepatocarcinoma in rat was established by diethylnitrosamine. Qinggan Huayu Peroral Solution in different concentration was given to investigate the expression of ATP enzyme in different groups by enzyme histochemisty and the expression of ALB, Notchl, Jagged by immunohistochemistry. Results： Compared with the model group levels of ATP enzyme and ALB were singnificantly increased （P〈0. 01）,and the levels of Notch l and Jagged were singnificandy decreased（P〈0.01）. Conelusion ：Qinggan Huayu Peroral Solution can prevent hepatic precancerous lesion rats,which is related to the regulating effect on Notch signaling system.

关 键 词：肝肿瘤 实验性/中医药疗法 癌前状态/中医药疗法 @Notch信号 

分 类 号：R285[医药卫生—中药学]