固相萃取HPLC测定人血清磷酸川芎嗪及其在药代动力学研究中的应用  

HPLC determination of ligustrazine phosphate concentrations in human serum with solid phase extraction and its pharmacokinetics studies

作　　者：胡晓[1] 李新华[2] 熊玉卿[1] 

机构地区：[1]江西医学院临床药理研究所,南昌330006 [2]江西医学院门诊部,南昌330006

出　　处：《药物分析杂志》2006年第12期1754-1756,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立磷酸川芎嗪人血清药物浓度测定方法,并用于磷酸川芎嗪人体药代动力学研究。方法:采用固相萃取高效液相色谱紫外检测法,样品经 Bond elut 固相萃取小柱处理。色谱柱为 Diamonsil C_(18)柱(4.6 mm×150 mm,5μm),流动相为:甲醇-水(62:38),三乙胺调 pH 为7.5,流速:1 mL·min^(-1)。检测波长280 nm。结果:磷酸川芎嗪血清浓度测定方法的线性范围为0.025～9.6μg·mL^(-1),线性关系良好(r=0.9999),最低定量限为0.025μg·mL^(-1)(以 S/N>4计),方法回收率大于90%,日内和日间 RSD 小于6%。结论:采用固相萃取高效液相紫外检测方法能快速可靠地测定磷酸川芎嗪血药浓度,适用于磷酸川芎嗪的血药浓度监测和人体药代动力学研究。Objective：To establish an HPLC with solid phase extraction method for the determination of concentration of ligustrazine phosphate in human serum and apply this method to the pharmacokinetic and bioavailability study of ligustrazine phosphate, nethods：Ligustrazine phosphate in serum was extracted with Bond elut solid phase extraction,HPLC instrument was used with C18 reversed -phase column and monitered by UV. The mobile phase consisted of methanol and water（62：38 ,pH =7.5） ,the eluated with a flow rate of 1 mL · min^-1 ,detection wave- length was 280 nm. Results：The calibration curve of ligustrazine phosphate in the range of 0. 025 -9. 6μg·mL^-1（r =0. 9999）. The recoveries of methodology were more than 90%. The intra - day and inter - day RSD were less then 6%. Conclusion ：This method has good sensitivity, precision and simple. It was shown to be suitable for pharmacokinetics studies of ligustrazine phosphate in humans and the clinical monitoring the serum concentration of ligustrazine phosphate.

关 键 词：磷酸川芎嗪 血药浓度 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]