HPLC-ELSD法测定磷脂的纯度和含量  

Purity analysis of phosphatidylcholine by HPLC-ELSD

作　　者：惠民权[1] 张琰[2] 傅经国 陈涛[1] 

机构地区：[1]西北工业大学生命科学院 [2]第四军医大学唐都医院 [3]西安力邦制药有限公司

出　　处：《药物分析杂志》2006年第12期1826-1828,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家863计划科技重大专项(2005AA2Z3G60);西安市生物医药与中药现代化专项(EX05007)

摘　　要：目的:测定磷脂辅料的纯度和含量。方法:以 YMC-PVA Silica 色谱柱为分析柱(柱温:常温),A:氯仿-异丙醇(50:50)和 B:氯仿-异丙醇-水(36:55:9)为流动相,采用梯度洗脱程序,流速1.0 mL·min^(-1),在蒸发光散射检测器(漂移管温度:40℃)下进行分析,进样量为20μL。结果:在1.0～1.60 mg·mL^(-1)范围内,磷脂对照品的峰面积(A)与其浓度(C)呈现良好的线性关系 A=7.717C-0.1652(R^2=0.9981)。最低检测限为1.7μg·mL^(-1),最低定量限为6.0μg·mL^(-1)。结论:本法用于测定磷脂辅料的纯度和含量快速、简便、专属性高、重现性好。Objective：To determine the content and purity of phosphatidylcholine by HPLC - ELSD. Methods：An HPLC method was developed. Phosphatidylcholine was chromatographed on a YMC - PVA Silica column and detected by ELSD （drift tube temperature at 40 ℃ ） and eluted with a gradient of CHClJ2 -PrOH（50： 50） to CHClJ 2 -PrOH/H2O（36： 55： 9）. Flow rate was 1.0 mL·min^-1 ,the injection volume was 20 μL. Resuits：A good linear relationship was found between peak area and the concentration of phosphatidylcholine over the range of 1, 0 - 1.6μg·mL^-1 （ R^2 = 0. 9981 ）. The LOD was 1.7μg·mL^-1 Conclusions： This method was rapid, convenient, selective and reproducible for determination of phosphatidylcholine and its purity.

关 键 词：磷脂 含量测定 纯度 HPLC—ELSD 梯度洗脱 

分 类 号：R917[医药卫生—药物分析学]