HPLC法测定银黄注射液中绿原酸的含量  

HPLC Determination of chlorogenic acid and baicalin in Yinhuang injection

作　　者：赵秀香[1] 李启艳 王磊 

机构地区：[1]山东省胸科医院,济南250013 [2]山东省药品检验所,济南250012 [3]烟台市药品检验所,烟台264000

出　　处：《药物分析杂志》2006年第12期1880-1882,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立银黄注射液中绿原酸、黄芩苷的含量测定方法。方法:采用高效液相色谱法,色谱柱:Thermo Hypersil BDS(4.6mm×250mm,5μm);紫外检测波长;绿原酸327nm,黄芩苷280nm;流动相:绿原酸为乙腈-0.3%磷酸(13:87),黄芩苷为乙腈-0.3%磷酸(23:77)。结果:绿原酸进样量在0.18～0.92μg范围内,黄芩苷在0.12～0.60μg范围内线性关系良好,平均回收率分别为97.7%和99.5%。结论:本方法准确、简便、快速,适合于该药的质量分析检验。Objective：To establish an analytical method of determinating baicalin in Yinhuang injection by HPLC. Methods：Chlorogenic acid in the injection was separated by HPLC on Thermo Hypersil BDS（4. 6 mm ×250mm,5μm）with a CH3CN-0. 3%H3PO4（ 13： 87）as mobile phase and detected at 327 nm,baicalin in the injection was separated by HPLC on Thermo Hypersil BDS （ 4. 6 mm ×250mm,5μm） with a CH3 CN - 0. 3 % H3 PO4 （ 23： 77 ） as mobile phase and detected at 280 nm. ResuLts： There was a good linear relationship with a range of 0. 18 -0. 92 μg for chLorogenic acid,0. 12 -0. 60 μg for baicalin ,the average recovery rate were 97.7% and 99.5% separately. Conclusion：The method is accurate, rapid and simple for quantitative analysis of Yinhuang injection.

关 键 词：银黄注射液 绿原酸 黄芩苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]