反相高效液相色谱法测定吊石苣苔中石吊兰素的含量  

RP-HPLC determination of nevadensin in Lysionotus pauciflorus Maxim.

作　　者：王绍云[1,2] 周光明[3] 黄成[3] 王莉[3] 高智席[3] 

机构地区：[1]凯里学院应用化学研究所 [2]西南大学化学化工学院,重庆400715 [3]西南大学化学化工学院

出　　处：《药物分析杂志》2006年第11期1617-1619,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：重庆市自然科学基金(CSTC-2004BB7087);黔东南民族师范高等专科学校(校科200505号)资助项目

摘　　要：目的:建立反相高效液相色谱法测定药用植物吊石苣苔(Lysionotus Pauciflorus Maxim.)中石吊兰素的含量。方法:用Hypersil C_(18)(4.6 mm×300 mm,5 μm)色谱柱,以甲醇-1%醋酸(85:15)为流动相,柱温25℃,335 nm 波长处以外标法测定。结果:石吊兰素在0.05～50.0 μg·mL^(-1)线性关系良好(r=0.9998),检测限(S/N=3)为0.026 μg·mL^(-1),平均回收率大于99%。结论:方法简单、可靠,可用于药用植物吊石苣苔中石吊兰素的测定。Objective： An HPLC method was established for the determination of nevadensin in Lysionotus pauciflorus Maxim. Methods：The conditions of chromatography： Hypersil C18 column（4. 6 mm × 300 mm ,5 μm） was adopted with a mobile phase of methanol -1% acetic acid（ 85：15 ） ,the column temperature was controlled at 25 ℃ and the wavelength of detector was set at 335 nm; external standard method was quantitative analysis method. Resuits： The linear range was 0. 05 -50. 0 μg · mL^- 1 （ r = 0. 9998 ） , The detection limit was 0. 026 μg · mL^- 1 （S/N = 3） ,the average recovery was over 99%. Conclusion ：The method has been successfully applied to the determination of nevadensin in Lysionotus Pauciflorus Maxim. ,and proves to be simple and reliable.

关 键 词：吊石苣苔 石吊兰素 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]