紫外分光光度法测定知母中总皂苷的含量  

UV determination of the total steroidal saponins in Anemarrhena asphodeloides Bge.

作　　者：薛小娟[1] 陈晓青[1] 李珏[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《药物分析杂志》2006年第11期1659-1662,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立知母总皂苷的提取和含量测定方法。方法:以75%的乙醇作提取剂,提取料液比为1:8,提取2次,每次超声提取30 min;以浓硫酸为显色剂,显色温度为60℃,显色时间为30 min,在283 nm 下用紫外分光光度法测定。结果:用紫外分光光度法测定总皂苷元的含量结果表明,总皂苷元含量在8.4×10^(-3)～5.04×10^(-2)mg·mL^(-1)之间与吸光度线性关系良好,平均回收率为98.92%,RSD 为0.30%,稳定性强,吸光度3 h 无明显变化。结论:超声提取法反应时间短,提取效率高。紫外分光光度法测定总皂苷简单方便,准确度和精密度均能满足分析要求。Objective： ：To establish method for determination of the total steroidal saponins in Anemarrhena asphodeloides Bge. Methods： Extraction twice with 75 % ethanol and 30 min every time, and the solid - liquid ratio was 1 ： 8 ; UV detection at 283 nm after reaction with sulphuric acid at 60 ℃ in 30 min. Results： In UV spectrophotometry Beer＇s law was obeyed within the range of 8.4 × 10^-3 -5.04× 10^-2 mg · mL^-1 ,the average recovery was 98.92%, with RSD of 0. 30%. The solution was stable in 3 hours. Conclusions：The ultrasonication assisted extraction is high efficient and convenient. The UV is simple, rapid and reproducible, and the results are reliable and accurate.

关 键 词：知母 总皂苷 超声提取 菝葜皂苷元 紫外分光光度法 

分 类 号：R917[医药卫生—药物分析学]