β-榄香烯脂质体的制备工艺研究  

Preparative processing technique of β-elemene liposomes

作　　者：黄汉昌[1] 朱宏吉[1] 张明贤[2] 李庆武[1] 林强[2] 兰天路[1] 

机构地区：[1]天津大学化工学院制药工程系,天津300072 [2]北京联合大学制药工程研究所,北京100023

出　　处：《中草药》2006年第12期1799-1802,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的确定β-榄香烯脂质体较佳的配方组成和制备方法。方法比较薄膜水化(TFH)、逆相蒸发(REV)和REV结合高压挤压法制备β-榄香烯脂质体方法,优选出较优的制备方法;光子相关衍射法测量β-榄香烯脂质体的粒径;气相色谱法测定包封率。结果薄膜水化法制备的β-榄香烯脂质体的包封率大于逆相蒸发法脂质体的包封率,但该法不易于批量生产。逆相蒸发法脂质体经高压挤压后在一定挤压程度内,粒径减小、包封率和载药量增加,所制得的β-榄香烯脂质体稳定性良好,6个月未发现凝絮分层现象。结论逆相蒸发结合高压挤压方法制备的β-榄香烯脂质体粒径小而分布较均匀,且有较高的载药量和包封率。Objective To optimize the composition and processing method of β-elemene liposomes. Methods The β-elemene liposomes were prepared by the thin film hydration （TFH）, the reverse-phase evaporation （REV）, and REV plus high pressure extrusion which were compared to select the eptimum preparative way. The envelopment rate of β-elemene was analyzed by GC. The particle diameter of β-elemene liposomes was measured by dynamic light scattering. Results Although the envelopment rate of β- ele-mene Liposomes prepared by TFH method was better than that prepared by REV method, it was difficult to be used to scale up. The envelopment rate and the load of β-ele-mene liposomes were increased while diameter of vesicles was decreased when prepared with REV plus high pressure extrusion. The obtained liposome had a good storage stability and not been found flocculated in six months. Conclusion The diameter β-elemene liposomes prepared by REV plus high pressure extrusion are smaller and uniform. The loading and envelopment rate of β-elemene is higher than that by traditional method.

关 键 词：Β-榄香烯 脂质体 高压挤压法 气相色谱 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]