丙酮酸钙对山羊瘤胃消化代谢的影响  

Effect of Calcium Pyruvate on Rumen Metabolism of Goats

作　　者：陈秀霞[1] 王小静[1] 王喜乐[1] 杨俊花[1] 沈向真[1] 陈杰[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《中国农学通报》2006年第12期17-20,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"共轭亚油酸在反刍动物体内形成的生物学机制与调控的研究"(30371040)

摘　　要：为探讨丙酮酸钙对山羊瘤胃消化代谢的影响。利用气相色谱法测定挥发性脂肪酸(VFA)含量;采用酚-次氯酸比色法测定NH3-N活性;考马斯亮蓝(G250)比色法测定瘤胃微生物粗蛋白(MCP)含量。结果发现动物试验和体外试验结果基本一致,丙酮酸钙不影响山羊瘤胃内环境pH值,提高乙酸含量(56.91±0.68vs61.68±0.79,P<0.01)降低瘤胃液中NH3-N浓度(3.620±0.24vs3.540±0.13,mM/L),促进蛋白质的生成(1135.81±34.92vs1225.05±49.47,mg/L)。所以添加丙酮酸钙对瘤胃的代谢有显著的促进作用。[Objective]To study the effects of Calcium Pyruvate on the digestion and metabolism of rumen. [MethodslThe volatility fatty acid （VFA） and NH3-N was measured by GC and colotimetry. The MCP content was measured by G250 colotimetry. [Results]The experiment was divided into two successive periods-in vitro and in vivo. Effect of Calcium Pyruvate on rumen digestion and metabolism in vitro was the same as in vivo. Calcium Pyruvate improves rumen digestion and metabolism, in, Calcium Pyruvate of 0.05g/kg BW/d did not change the pH of ruman, and increased the content of acetate （56.91 ±0.68 vs 61.68±0.79, P〈0.01）, reduced the rumen degradation of dietary protein （1135.81 ±34.92 vs 1225.05± 49.47, mg/L）,also reduced the concentration of NH3-N （3.620±0.24 vs 3.540 ±0.13,mM/L）. Conclusion Calcium Pyruvate can effect on rumen metabolism.

关 键 词：丙酮酸钙 瘤胃 消化代谢 

分 类 号：S827[农业科学—畜牧学]