单平台TruCount和PLG-CD_4测定CD_4T细胞绝对计数的比较  

Comparison of CD_4 T cell counts determined by single platform TruCount and PLG-CD_4 gating technologiy

作　　者：徐建青[1] 彭虹[1] 任莉[1] 袁霖[1] 绳波[1] 阮玉华[1] 韩丽凤[1] 高冰 谷永斌[3] 丁心平[4] 徐臣[4] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050 [2]颖州区疾病预防控制中心,安徽阜阳236000 [3]阜南县疾病预防控制中心,安徽阜南236300 [4]阜阳市疾病预防控制中心,安徽阜阳236000

出　　处：《中国艾滋病性病》2006年第5期393-395,共3页Chinese Journal of Aids & STD

基　　金：中国CIPRA项目资助(U19AIS1915-03);国家"十五"科技攻关课题资助(2004BA719A06-02)

摘　　要：目的了解单平台TruCount和PLG-CD4两种不同流式设门方法,对同一样品测定CD4T细胞绝对计数的影响。方法采集408份样品,包括同批次114份,不同时间15批次294份,比较两种不同设门方法检测同一样品CD4T细胞的绝对计数。结果对TruCount和PLG-CD4两种流式设门方法所获取的408份样品的CD4T细胞绝对计数进行分层后显示:只有4例在分层时落入不同范围,判定差异率<1%;在≤200个细胞/μl的范围内,PLG-CD4判定90例,比TruCount少判1例。两种不同方法所得CD4T细胞绝对计数差异均<10%,相关系数r=0.99。结论单平台TruCount和PLG-CD4两种不同流式设门方法测定的CD4T细胞绝对计数是一致的,实际应用中所采用的方法将决定于不同试剂的价格与操作的简易性。Objective To compare CD4T cell counts determinaed by single platform TruCount or PLG CD4 gating technology for specimens sampled at one time point or at different times points. Methods Four hundred and eight specimens including 114 sampled at one time and 294 .sampled at 15 different times points were tested by both single platform TruCount and PLG gating technologies to quantify CD4T cell counts. Results When their CD4T cell counts were stratifled as ≤200,201 - 500 and ≥500 cells/μl,only 4 out of 408 samples（ 〈 1% ）were ranged differently between TruCount and PLG CD4 gating. At the range of CD4T cell counts ≤200 cells μl, determined PLG-CD4 90 HIV cases were iolentified by PLG CD4, i. e. ,one less than cases determined by TruCount.Only 〈 10% differences were observed between CD4T call counts dctermined by TruCjount and PLG-CD4 gatings. Overall,CD4T cell counts determined by two technologies are highly correlated（ r = 0.99）. Conclusion No significant differences were observed in CD4T cell counts determined by single platform TruCount and PLG-CD4 gating technologies. The price of reagents and simplicity of their pactical application will be the major consideration for technolog cal options in resource constrained settings.

关 键 词：艾滋病病毒 TruCount PLG-CD4 CD4细胞绝对计数 

分 类 号：R466.63[医药卫生—临床医学]