甘露糖结合蛋白基因多态性与肺结核发病关系的研究  

Study on mannose-binding protein gene polymorphisms and susceptibility to pulmonary tuberculosis

作　　者：冯福民[1] 郭梅[1] 刘茜[1] 王东[1] 高宝霞[1] 孙永红[1] 安雅臣[1] 纪春梅[1] 

机构地区：[1]华北煤炭医学院流行病与卫生统计学科,唐山063000

出　　处：《中华流行病学杂志》2006年第12期1082-1085,共4页Chinese Journal of Epidemiology

基　　金：河北省卫生厅基金资助项目(04256);河北省教育厅博士基金资助项目(B2004127)

摘　　要：目的探讨甘露糖结合蛋白(MBP)基因多态性与中国人肺结核发病的关系。方法采用病例对照研究,收集肺结核病例125例和健康对照198名的环境因素暴露情况及静脉血,MBP基因多态性分析采用特异引物PCR方法,用SPSS 11．5软件进行危险因素的单因素分析和多因素非条件logistic回归分析。结果病例组和对照组MBP-52、54、57位点的等位基因突变频率分别为8．0％、7．2％、0．4％和5．3％、4．3％、0．5％,按性别分层的Mantel-Haensezl X2分析两组间差异无统计学意义。单因素分析表明体重指数、婚姻状况、卡介苗接种史、卡痕、结核病接触史及家族史、体力劳动、文化程度与肺结核的发生有关。在多因素分析中调整上述危险因素后,总MBP(OR=2．182,95％CI: 1．058-4．499)和MBP-52突变型等位基因型(OR=2．574,95％CI:1．028-6．446)与肺结核发病有统计学意义。结论总MBP和MBP-52突变型等位基因可能与肺结核发病有关。Objective To explore the association between the genetic polymorphisms of mannosebinding protein （MBP） alleles and suseeptibility to pulmonary tuberculosis, Methods 125 pulmonary tuberculosis cases and 198 healthy controls were collected. A case-control study was conducted. Three structural gene mutations in exon 1 of MBP gene （codon 52, codon 54 and codon 57） were studied. Polymerase chain reaction with sequence-specific primers （PCR-SSP） was carried out in the polymorphism in MBP alleles. Information on related risk factors of tuberculosis was collected, using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package. Results The frequencies of mutant heterozygote or homozygote of MBP 52,54,57 were 8.0 % ,7.2 % and 0.4% for cases and 5.3 % ,4.3% ,0.5 % for controls,respectively. The distribution of mutant genotypes of MBP did not show significant difference between tuberculosis patients and control by Mantel-Haenszel X^2 on sex.The univariate analysis demonstrated that body mass index, marital status, vaecinal vestige, bacillus of Calmette-Guerin vaccine immunization, contacted with pulmonary tuberculosis patients, familial traits were the risk factors of pulmonary tuberculosis, After adjusting those related environmental factors in the multivariate logistic analyaes, the total MBP（ MBP-52, MBP-54 and MBP-57） and MBP 52 heterozygote genotypes were significantly overrepresented in cases, with adjusted OR （95% CI） being 2. 182 （1.058- 4. 499） and 2.574 （ 1. 028- 6.446）, Conclusion Total MBP and MBP-52 mutant genotypes might be associated with the susceptibility to pulmonary tuberculosis.

关 键 词：结核 肺 甘露糖结合蛋白基因 病例对照研究 基因多态性 

分 类 号：R521[医药卫生—内科学]