周围血单个核细胞抑制胰岛素释放的实验研究  

A study on the suppression of insulin release by peripheral blood mononuclear cells from IDDM patients in vitro

作　　者：崔国敏[1] 陈茜[1] 郭长江 欧阳安 

机构地区：[1]山东省济宁医学院附属医院内分泌科,河南医科大学,省医学科学研究所肝炎组

出　　处：《中国糖尿病杂志》1996年第4期212-214,共3页Chinese Journal of Diabetes

摘　　要：将新发病的ＩＤＤＭ病人周围血单个核细胞（ＰＢＭＣ）同大鼠胰岛共同培养２０个小时后用Ｌ－精氨酸刺激，收集并测定基础和刺激后培养上清中胰岛素的含量，结果显示：ＩＤＤＭ病人ＰＢＭＣ作用下的基础胰岛素释放（１１７．９±１４．０±μＵ·１０ｉｓｌｅｔｓ－１／２０ｈ）（ｎ＝１１）和刺激后胰岛素释放（１４７．５±３２．３μＵ·１０ｉｓｌｅｔｓ－１／３ｈ）（ｎ＝１１），显著低于正常人ＰＢＭＣ作用下的基础胰岛素释放（１８４．８±２９．５μＵ）（ｎ＝１０，Ｐ＜０．０１）和刺激后胰岛素释放（１９５．０±２７．４μＵ）（ｎ＝１０，Ｐ＜０．０１）。结果表明新发病的ＩＤＤＭ病人ＰＢＭＣ能够抑制大鼠胰岛基础和刺激后胰岛素的释放。The pancreatic islets isolated from rats by collegenase, and purified by Ficoll-Densitygradient-centrifugation,were incubated for about 20 hrs in DMEM with peripheral blood mononuclear cells(PBMCs)of newly onset IDDM.Then,the islets were stimulated by L-arginine(10mmol/L)in DMEM.The supernatant were taken before and after stimulation for detecting insulin by radioimmunoassay as basic and stimulated insulin release.The results were as follows:In the presence of PBMCs from IDDM patients:the mean basic insulin release was 117.9±14.0μU·10 islets-1/20h(n=11).It was significantly lower than corresponding results(184. 8±29.5μU·10 islets-1/20h)(n=10,P<0.01)in the presence of control PBMCs.The mean stimulated insulin release of 147.5±32.3μU·10 1slets-1/3h from IDDM group was significantly lower than the corresponding release of 195.0±27.4μU ·10 islets-1/3h of the control group(P<0.01).Our results display that the PBMCs of IDDM may inhibit the basic and stimulated insulin release of islets, and demonstrate that the cell-mediated immunity disturbance may play a role in the pathogenesis of IDDM.

关 键 词：糖尿病 胰岛素依赖型 周围血单个核细胞 胰岛素释放 

分 类 号：R587.102[医药卫生—内分泌]