乳糖作为诱导剂对人β-防御素3蛋白表达的影响  

The Influences of Lactose as an Inducer on Expression of Human β-defensin 3 in E.coli

作　　者：李春丽[1,2] 席燕燕[1] 陈正华 何国庆[4] 

机构地区：[1]河南农业大学牧医工程学院 [2]浙江大学生物系统工程与食品科学学院杭州310019 [3]甘肃亚盛集团博士后科研工作站北京分站 [4]浙江大学生物系统工程与食品科学学院

出　　处：《微生物学通报》2006年第6期84-88,共5页Microbiology China

基　　金：河南省自然科学基础研究项目(No.0411030300);河南省教育厅基础研究项目(No.2004922043)

摘　　要：对人β-防御素3基因工程菌株BL21-pET-hBD3的乳糖诱导条件进行了研究。乳糖浓度、诱导时间和诱导温度对菌株生长和目的蛋白表达的试验结果显示,高浓度乳糖对菌株生长有抑制作用(P<0.01),对目的蛋白的表达无显著影响(P>0.05),延长诱导时间对菌株生长有利(P<0.01),但对蛋白的表达无显著影响(P>0.05)。以0.5%乳糖在37℃诱导4h对目的蛋白的表达有利,目的蛋白的含量可达28.1%。控制好诱导条件,乳糖与IPTG的诱导效果基本相当。The inductive conditions used lactose as inducer for the flask-shaking of E. coli BL21-pET-hBD3 have been studied. The effect of three factors which were lactose concentration , induction time and temperature on growth of strains and on the yield of hBD3 was analyzed in detail. The result indicated that high lactose concentration inhibit the growth of strains （ P 〈0.01 ） but had little effect on expression of target protein among 0. 5% -5% （ P 〉 0.05 ） , Biomass would be improved as time passed （ P 〈0. 01 ） , but the yield of expressed protein didn＇ t increase obviously （ P 〉 0. 05 ） . The greater expressed level of hBD3, as high as 28.1% of total strain protein, could be gained when strain was induced by 0. 5% lactose at 37℃ for 4h. The effect using lactose as inducer is almost as same as using lPTG as inducer under control conditions.

关 键 词：人β-防御素3 大肠杆菌 重组表达 优化 

分 类 号：Q786[生物学—分子生物学]