控制再灌注流量对供体肺功能影响的实验研究  

Influence of Controlled Reperfusion Flow Rate on Donor Lung

作　　者：杨研[1] 禹亮[2] 王巨[2] 王柏春[2] 宋微[2] 

机构地区：[1]中国协和医科大学中国医学科学院心血管病研究所阜外心血管病医院外科,北京100037 [2]哈尔滨医科大学附属第一医院胸心血管外科

出　　处：《中国伤残医学》2006年第4期1-3,共3页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:研究控制再灌注流量对大鼠供体肺功能的影响。方法:应用大鼠离体肺灌注模型再灌注大鼠供体肺,分3组:低流量组以4ml/min的流量灌注30分钟;高流量组以8ml/min的流量灌注30分钟;控制流量组,先以4ml/min流量灌注供体肺5分钟,后以8ml/min的流量继续灌注25分钟。测定血氧分压,取肺组织测定湿干比及观察超微结构改变。结果:A组的各项指标均明显好于B组和C组(P<0.05);同时,C组各项指标较B组均有明显改善(P<0.05)。结论:再灌注开始早期控制灌注流量可减轻缺血再灌注损伤,改善移植肺功能。Objective： To study the effects of controlled reperfusive flow rates on the function of rat preserved lung. Methods： The rat donor lungs were reperfused using an isolated, whole blood-perfused, ventilated rat lung model. The lungs were randomly divided into 3 groups： Low flow group underwent reperfusion at 4 ml/min for 30 minutes; high flow group underwent reperfusion at 8 ml/min for 30 minutes; controlled flow group underwent initial reperfusion at 4 ml/min for 5 minutes, followed by reperfusion at 8 ml/min for 25 minutes. Oxygenation, wet-to-dry ratio of grafts tissue was measured, and ultrastructure were observed. Results： Group A had significantly improved arterial oxygenation, wet-to-dry weight ratio measurements and ultrastructure compared with＇ groups B and C after 30 minutes of reperfusion（P〈0.05）. However, Group C had much improved measurements compared with Gre.up B（P〈0.05）. Conclusion： Controlled reperfusion using a low initial flow rate might decrease the severity of reperfusion injury associated with high-flow rates.

关 键 词：器官保存 缺血再灌注损伤 肺移植 

分 类 号：R114[医药卫生—卫生毒理学]