不同光周期条件下PhyB和ABA对不同苜蓿品种的秋眠性调控  

Regulation of PhyB and ABA on fall dormancy of different Medicago sativa varieties in photoperiod treatments

作　　者：王成章[1] 韩锦峰[2] 胡喜峰[1] 张春梅[1] 何云[1] 刘圈炜[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南农业大学农学院,河南郑州450002

出　　处：《草业学报》2006年第6期56-63,共8页Acta Prataculturae Sinica

基　　金：国家农业科技成果转化资金项目;河南省科技厅重大科技攻关项目(0422010300)资助

摘　　要：短日照和低温是导致苜蓿秋眠的主要环境因子。本研究检测了3个不同秋眠型苜蓿在8,12和16 h光照光周期条件下叶片和顶芽中PhyB和ABA的含量,并探讨其对秋眠的影响机制。结果表明,1)和非秋眠苜蓿相比,秋眠苜蓿叶片中PhyB含量高,PhyB作为绿色植物主要光受体可能通过光周期调控或参与了苜蓿的秋眠;并且3个品种均是在8 h光照下PhyB合成量高,暗示长暗期利于其合成。2)和12,16 h光照相比,8 h处理顶芽和第一展开叶中ABA含量最高,显然短日照利于ABA的合成;8 h光照处理时,秋眠型苜蓿品种Vernal ABA含量分别比半秋眠型CW5 0 2和非秋眠型CUF1 0 1高2.1 2和2.0 3倍,1 2 h光照处理ABA含量Vernal比CW5 0 2和CUF1 0 1分别高3 6.2 4%和46.46%,但16 h光照处理ABA含量并不表现上述规律,说明8和12 h光照程度不同地为苜蓿创造了逆境效应,ABA可能是调控或诱导秋眠的激素。3)8 h光照下苜蓿秋眠特征明显,生长严重受抑,不开花;12 h光照下苜蓿秋眠特征比8 h光照处理轻,生长受抑但抑制程度低于8 h光照处理,不开花;16 h光照下3个品种生长、开花正常。可能是PhyB含量多寡程度不同地调控了光周期反应,进而影响了ABA合成,而ABA在不同光周期诱导下通过合成量不同调控了不同秋眠基因表达,诱发了秋眠。Short days and low temperature were the main environmental factors causing fall dormancy in M. sativa. The experiment was conducted in the laboratory with three M. sativa varieties of different fall dormancy types and with three different photoperiods to study regulation of PhyB and ABA on fall dormancy in M. sativa. Major results were： 1）PhyB content in the leaves of Vernal was higher than that in CW502 and CUF101, and PhyB content in SD was higher than in MD and LD indicating that the dark phase was more important than the light phase in fall dormancy than in non-fall dormancy for the synthesis of PhyB. 2） In the buds and top unfolding leaf of shoots of each variety, ABA content had a similar pattern to that of PhyB, hinting that ABA was the main hormone regulating fall dormancy in M. sativa. 3） In SD and MD, the PhyB and ABA were increased and growth was inhibited so they did not bloom at the same time. It is thought that PhyB was main photoreceptot of photoperiod for fall dormancy in M. sativa. Increased PhyB of the leaves in fall dormancy M. sativa resuited in improved ABA content of the buds so that gene expression of fall dormancy was guided and enhanced. In the long day LD condition, the PhyB and ABA decreased, growth was not inhibited and plants could bloom normally.

关 键 词：苜蓿秋眠性 短日照 PHYB ABA 

分 类 号：S541.9[农业科学—作物学]