Fibrillin操纵子在甘蓝型油菜和拟南芥中的瞬时表达  

Transient Expression of Fibrillin Promoter in Protoplasts of Arabidopsis thaliana and Brassica napus

作　　者：滕蕾[1] 黄瑾[1] 赵竹露[1] 李旭锋[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2006年第6期1394-1398,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金资助(项目批准号:30470927)

摘　　要：将Fibrillin操纵子驱动的荧光素酶报告基因表达载体导入拟南芥原生质体中,研究基因表达调控,证明脱落酸(ABA)诱导报告基因的表达.同时将相同的质粒转化到甘蓝型油菜蜀杂9号原生质体中,获得与拟南芥中类似的表达结果.实验结果说明Fibrillin操纵子在拟南芥和油菜原生质体中均对ABA的诱导敏感.Arabidopsis fibrillin promoter was constructed in pBI221, and the plasmid was transferred into protoplasts of Arabidopsisor Brassicavia PEG-mediated method, showing a transient expression. After adding 30μmol/L ABA, it was found that the expression was upregulated, indicating fibrillin is sensitive to ABA in both Arabidopsis thaliana and Brassica napus.

关 键 词：Fibrillin启动子 荧光素酶 PEG介导的转化 瞬时表达 脱落酸 

分 类 号：Q78[生物学—分子生物学]