麻疯树毒蛋白(curcin)基因转化水稻的研究  

Study on Transformation of Rice for Curcin Gene of Jatropha curcas

作　　者：廖毅[1] 魏琴[1] 徐莺[1] 王胜华[1] 唐琳[1] 白洁[1] 陈兴春[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2006年第6期1399-1404,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国际合作项止"中国西南地区能源植物的开发和利用"(No.2004DFB00300)

摘　　要：以粳稻(Japonica)品种日本晴与中花11号为材料,对影响农杆菌介导水稻转化体系的几个因素进行了研究,建立了农杆菌介导的有效水稻转化体系.在该体系中,首次使用浓度为10-4g/mL嘌呤合成抑制剂acivicin在转化前对水稻愈伤组织进行预处理,结果表明此法可有效提高GUS基因的瞬时表达率.同时,利用插入了麻疯树毒蛋白(curcin)基因的植物表达质粒pBI121-curcin,通过农杆菌介导转化水稻,获得了多株转基因植株.通过PCR鉴定,证实curcin基因已整合到水稻基因组中.Several important factors have been investigated on affecting Agrobacterium-mediated rice transformation system by using rice cultivars Nipponbare and Zhonghua No. 11, and an efficient Agrobacterium-mediated rice transformation system was developed. In this system, it was found that the GUS transient expression of transformed rice calli was improved by prior exposure to 10^-4g/mL acivicin, which is a kind of purine synthesis inhibitors, before infection with A. tumefaciens. At the same time, using the plasmid pBI121- curcin which carried curcin gene of Jatropha curcas, transgenicrice plants were obtained by the Agrobacterium-mediated rice transformation system. Most transgenic plants were identified by PCR ananlysis and the results indicated that erucin gene had been integrated into the genome of transgenic rice plants, which provide an efficient way for transferring useful foreign genes to rice plants and lay the basis for the further study on the function of curcin gene.

关 键 词：水稻 农杆菌 转化 ACIVICIN 麻疯树毒蛋白基因 

分 类 号：Q78[生物学—分子生物学]