去势对离体大鼠心脏缺血／再灌注心功能及心肌凋亡的影响  

Effects of orchiectomy on the post-ischemic cardiac functional recovery and myocardial apoptosis in rats

作　　者：张中[1] 郑强荪[1] 马恒[2] 陈迈[3] 高渊[1] 雷靖[1] 张超[1] 刘文秀[1] 张宪伟[1] 

机构地区：[1]第四军医大学唐都医院心血管内科,陕西西安710038 [2]第四军医大学基础部生理教研室,陕西西安710032 [3]第四军医大学西京医院心脏内科,陕西西安710032

出　　处：《现代生物医学进展》2006年第11期11-13,共3页Progress in Modern Biomedicine

摘　　要：目的:观察去势对离体大鼠心脏缺血／再灌注心脏功能和心肌凋亡的影响。方法:SD大鼠28只,随机分为去势组、对照组,每组14只。制备大鼠离体缺血／再灌注模型(缺血30min,再灌注2h),观察左室压力,再灌注结束后检测心肌梗死率和细胞凋亡指数,免疫组化技术检测心肌组织的Bcl-2、Bax水平。结果:(1)与对照组相比,去势组再灌注后的心脏左室收缩及舒张功能无显著变化(P>0．05);(2)去势组心肌梗死范围(43．68±6．89％)较对照组(39．33±7．85％)增加,但无统计学差异;(3)对照组和去势组间心肌细胞凋亡指数无统计学差异(P>0．05),心肌组织中Bcl-2、Bax含量未见显著变化。结论:去势对心脏缺血／再灌注心脏功能没有保护作用,而且不影响心肌细胞凋亡过程,生理剂量的雄激素对缺血再灌注后的心脏功能不产生损害作用。Objective： To study the effects of orchiectomy on the post-ischemic cardiac recovery and myocardial apoptosis in rats. Methods： Nonorchiectomized mature 1hale SD rats were randomly assigned to control （n=14） and orchiectomized group （n=14）. After 2 weeks of treatment, the hearts were rentoved and placed in a Langendorff setup. The isolated, buffcr-perfosed hearts were subjected to occlude the left anterior descending coronary artery for 30 min and 120 min of reperfusion. Recovery of myocardial function was measured by analyzing pre and post-ischemlc left ventricular （LV） systolic/diastolic pressure simultaneously, and myocardial infarction and cardiac myocyte apoptosis were determined at the end of reperfusion.Bcl-2 and Bax protein were analyzed by lmmunohistochemical method. Results： The recovery of contractile function was not difference in two groups at the end of the reperfusion, neither LV systolic pressure nor end-diastolic pressure, No significant differences could be observed in the different experimental groups regarding both in myocardial infarction （orchiectomized group 43.68±6.89% vs control group 39.33±7.85%） and apoptosis index （orchiectomized group 6.5±2.3% vs. control group 5.8±0.9%）, and in the cxpression of Bcl-2 and Bax protein, there was no significant change between the groups, Conclusion： Orchiectomy has no protective effect on ischemia/reperfusion rats heart contractile function, and the physiological dose of androgen（such as testosterone ） has no harmful effect on the acute I/R heart.

关 键 词：去势 雄激素 缺血/再灌注 心肌梗死 凋亡 

分 类 号：R541.4[医药卫生—心血管疾病]