从人的肝癌组织中快速分离纯化γ-谷氨酰转移酶  

Rapid purification of γ-glutamyltranspeptidese from human liver cancer tissue

作　　者：李勇[1] 王念跃[2] 赵伟[2] 孟泽[1] 史力宁[1] 宋熠春[3] 严明[4] 

机构地区：[1]南京军区南京总医院医学检验中心,江苏南京210002 [2]东南大学医学院附属南京第二医院,江苏南京210003 [3]江苏大学医学技术学院,江苏镇江212001 [4]南京工业大学制药与生命科学院,江苏南京210008

出　　处：《中国生化药物杂志》2006年第6期326-328,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：南京市医学重点科技发展项目(No.ZKX0303)

摘　　要：目的应用KTATMexplorer 100蛋白质快速色谱纯化系统从人肝癌组织中分离纯化特异性γ-谷氨酰转移酶(GGT),为制备其抗血清提供抗原材料,以便对肝癌早期诊断进行研究。方法人肝癌组织经匀浆、离心、溶解、透析和色谱等步骤进行纯化;纯化过程中用国际临床化学学会推荐方法测定GGT催化活性,用紫外法监测蛋白质出峰图谱,考马斯亮蓝法测定蛋白质含量。结果人肝癌组织经过粗提、疏水色谱、DEAE阴离子交换色谱等纯化步骤得到电泳纯的GGT,其比活为0.36 U/mg蛋白质,纯化倍数为24,回收率为5.0%。结论该纯化方法能较好且有效地分离纯化肝癌特异性GGT。Purpose To purify γ-glutamyltmaspeptidase from human liver cancer tissue by AKTA^TM explorer 100 rapid protein chromatography system, which is then used to produce the antibodies targeted for early diagnosis of liver cancer. Methods Human liver cancer tissue is purified by rough extraction, dialyzation, chromatography etc. Results The CGT in human liver cancer tissue was purified. The catalysis units per milligram protein of CCT is 0.36 U and it has been increased about 24 times. And the yield of the CGT was 5.0% . Conclusion This protocol is efficient to purity GGT from human liver cancer tissue.

关 键 词：Γ-谷氨酰转移酶 人肝癌组织 色谱 纯化 抗原 

分 类 号：R735.7[医药卫生—肿瘤]