昆明山海棠总生物碱诱导肺腺癌A549细胞凋亡与细胞周期改变  

Effect of alkaloids from tripterygium hypoglaucum hutch on apoptosis of human lung adenocarcinoma cell line A549

作　　者：刘乐斌[1] 刘胜学[1] 胡孝贞[2] 杨录军[1] 敖琳[1] 周燕虹[1] 黄晓春[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室重庆市卫生毒理学实验室,重庆400038 [2]解放军第181医院B超室,桂林541002

出　　处：《第三军医大学学报》2007年第1期18-20,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30100241)~~

摘　　要：目的为探讨昆明山海棠总生物碱(alkaloids from Tripterygium Hypoglaucum Hutch,THHa)抗肺癌机制,研究THHa诱导肺腺癌A549细胞凋亡与细胞周期改变的关系。方法应用形态学观察、DNA凝胶电泳、流式细胞仪检测分析肺腺癌A549细胞凋亡及细胞周期改变。结果THHa能够诱导肺腺癌A549细胞凋亡并主要作用于细胞周期S期(S期细胞增多)。结论THHa可能通过诱导细胞凋亡而抑制A549细胞增殖,这种作用与细胞周期有关,这些有助于将来肺癌治疗方案的合理设计。Objective To explore the mechanism of the alkaloids from tripterygium hypoglaucum hutch （THHa） on the apoptosis of lung cancer. Methods Apoptosis and cell cycle of human lung adenocarcinoma cell line A549 were detected by morphological observation, DNA electrophoresis, and flow cytometry. Results THHa could induce apoptosis of human lung adenocarcinoma cell line and arrest the cells at S phase of cell cycle. Conclusion Apoptosis contributes to inhibitory effect of THHa on A549 cells. This may be helpful in designing an appropriate chemotherapeutic regimen in future.

关 键 词：昆明山海棠 肺腺癌A549细胞 细胞凋亡 细胞周期 

分 类 号：R282.71[医药卫生—中药学] R73-361[医药卫生—中医学]