北美悬铃木的组织培养  

Tissue Culture and Mass Propagation of Platanus occidentalis Linn

作　　者：邹永梅[1] 施季森[1] 

机构地区：[1]国家林业局江苏省林木遗传和基因工程重点实验室(南京林业大学)

出　　处：《南京林业大学学报（自然科学版）》2006年第6期61-65,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局2003年度重点课题

摘　　要：从北美一球悬铃木截冠植株上采当年生萌蘖枝条作为起始外植体进行组培试验,当用其茎段作为外植体诱导腋芽时,MS+NAA(0.1 mg/L)+6 BA(1.0 mg/L)+蔗糖(30 g/L)为适合的培养基,而在系统研究基本培养基、植物激素等因素对北美一球悬铃木不定芽增殖的影响后发现:以DCR为基本培养基,添加NAA(0.1 mg/L)+6 BA(1.0 mg/L)+KT(0.3 mg/L)+蔗糖(30 g/L)可促进北美一球悬铃木不定芽的增殖,产生的不定芽生长情况良好、增殖系数高,是比较适合芽增殖的培养基。生根培养时,笔者在实验中设计了两条生根技术路线:一步法生根,将芽直接接种在含添加物R(150 mg/L)的DCR培养基上;二步生根法,先将芽接种在含添加物R(60 mg/L)的DCR培养基上15 d左右,再转接至DCR基本培养基上。结果表明两条生根培养途径下的生根率均达100%。Top cut pruning was made on an American sycamore crown, and segments from new sprout were collected as tissue culture initial explants. The results indicated that as for shoot segments induction buds the optimal medium was MS＋NAA（0. 1 mg/L）＋6 -BA （1.0 mg/L）＋sucrose（30 g/L）, and DCR＋ NAA （0. 1 mg/L） ＋ 6 - BA（1.0 mg/L） ＋ KT （0. 3 mg/L）＋sucrose（30 g/L） was the optimal medium for induction buds proliferation, on which induction buds grew well and coefficient of multiplication reached 6.4 times. We developed two ways to get adventitious buds to root. One was that adventitious buds were transferred on the DCR medium with 150 mg/L R. The other, on the DCR medium with 60 mg/L R about for 15 d and then, transferred on a garniture-free DCR. By the two ways, both of the rooting percentages were up to 100%.

关 键 词：北美悬铃木 组织培养 外植体 

分 类 号：S722[农业科学—林木遗传育种]