蓝色犁头霉JY-2^#的选育与发酵特性  

Breeding and Fermentation of Absidia coeralea JY-2^# for Hydrocortisone Biotransformation

作　　者：赵荣[1] 王敏[1] 骆健美[1] 杜连祥[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院

出　　处：《天津科技大学学报》2006年第4期44-47,共4页Journal of Tianjin University of Science & Technology

摘　　要：将经过紫外线诱变的蓝色犁头霉原生质体倾注于含有化合物RSA(17α-羟基孕甾-4-烯-3,20-二酮-21-醋酸酯)的高渗再生培养基平板上,选育具有RSA抗性的氢化可的松生产菌株。获得60株抗性突变株,其中8株突变株的氢化可的松转化率高于出发菌株。突变株JY-2#的氢化可的松转化率达81.63%,较出发菌株提高了14.1%。初步研究了突变株JY-2#在5 L发酵罐的转化工艺和转化反应动力学。A method of compound RSA （ 17 α-hydroxy-pregn-4-en-3,20-dion-21-hydrate） resistance screening was applied to breed mutants with hydrocortisone（HC） transfotmation. The protoplast of Absidia coeralea, treated with UV light were regenerated on agar plates containing compound RSA and 60 resistance mutants were obtained. The HC conversion rates of 8 mutants were higher than that of the original strain. Among them, the HC conversion rate of mutant JY-2^# reached 81.63%, it was 14.1% more than that of the original strain. The fermentation conditions and the fermentation course were studied.

关 键 词：诱变育种 抗性突变株 蓝色犁头霉 氢化可的松 

分 类 号：Q93-335[生物学—微生物学]