Al Cr胁迫对菊芋叶片过氧化物同工酶的影响  

Effects of Chromium and Aluminum Stress on Peroxidase Isozyme in Leaves of Jerusalem Artichoke(Helianthus tuberosus.)

作　　者：陈燕珍[1] 胡丽莲[1] 陶毅明[1] 梁杨琳[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《农业环境科学学报》2006年第6期1427-1431,共5页Journal of Agro-Environment Science

基　　金：广西师范大学生态学博士点建议项目(XKY2006ZD02)子课题

摘　　要：对室内栽培的菊芋(Helianthustuberosus)幼苗分别经50、100、200μmol.L-1不同浓度的铝(Al3+)和铬(Cr6+)处理后,用聚丙烯酰胺凝胶电泳(PAGE)法分离其叶片过氧化物酶(peroxidase,POD)同工酶,测定了株高,比较了与自然条件生长的菊芋幼苗不同器官POD同工酶的特点及不同生育期POD活性的变化规律。结果表明,不同浓度的Al3+、Cr6+处理菊芋幼苗后,叶片共有7条POD同工酶带(P1～P7),比对照新增了2条(P1、P2)酶带,并随着处理浓度增加,酶带颜色加深,酶活性增大。不同浓度的Al3+、Cr6+处理,都能不同程度地抑制菊芋幼苗植株的生长,Cr6+的抑制作用大于Al3+。自然条件生长的菊芋幼苗不同器官各有特征酶带,叶片有5条POD同工酶带(P3～P7),未见P1、P2酶带出现。开花期叶片POD酶活性最高,苗期和成熟期的活性较低。Under the treatment of 50,100 and 200 μmol·L^-1 Al^3＋ and Cr^6＋ in greenhouse, the peroxidase （POD） isoenzymes in Jerusalem artichokes （Helianthus tuberosus ）plant leaves were analyzed by PAGE and the height of seedlings was measured to study the plants tolerance to aluminum and chromium. POD isoenzymes in different organs at seedling stage and changes of POD activity during different growth periods were compared in natural environment. The results showed that compared with the control, 7 bands of POD-isoenzymes （P1-P7）were observed in leaves of He lianthus tuberos us s seedlings after Al^3＋. and Cr^6＋ treatment, including 2 new bands （P1 and P2）. The enzyme activities increased as the concentration of Al^3＋ and Cr^6＋ increasing with the intensified band color. The plant growth was inhibited by Al^3＋ or Cr^6＋ to some extent, but the inhibition effect of Cr^6＋ was more remarkable. The POD-isoenzyme bands varied with organs of Helianthus tuberosus seedling, and 5 bands of POD-isoenzymes in leaves were observed （P3-P7）without P1 or P2. Anthesis plants had higher POD activities than the seedlings or mature plants. These results provided reference for studying Ilelianthus tuberosus tolerance to Al^3＋ and Cr^6＋.

关 键 词：菊芋 AL CR 过氧化物酶 同工酶 耐性 

分 类 号：X503.233[环境科学与工程—环境工程]