移植肾组织Fas配体和T细胞内抗原-1表达水平的定量分析  

Quantitative determination of Fas ligand and T-cell intracellular antigen-1 in renal allografts

作　　者：张万君[1] 梅长林[1] 叶朝阳[1] 赵学志[1] 孙田美[1] 

机构地区：[1]第二军医大学长征医院肾内科,上海200003

出　　处：《解放军医学杂志》2006年第12期1175-1177,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究移植肾组织Fas配体(FasL)和T细胞内抗原-1(TIA-1)的定量检测及临床意义。方法取移植肾组织32例、对照标本10例,采用竞争性RT-PCR技术分别检测FasL和TIA-1的表达,结果以靶基因/β-actin的比值表示,并与根据Banff标准进行的病理学诊断比较分析。结果将标本分为急性排斥(14例)、慢性排斥(15例)和无排斥改变(13例)三组,其FasL表达例数分别为8例、3例和0例,TIA-1表达例数分别为14例、10例和3例。急性排斥组FasL和TIA-1水平明显高于慢性排斥组(P<0.05)和无排斥组(P<0.05),而慢性排斥组与无排斥组比较无显著性差异(P>0.05);急性排斥组FasL和TIA-1的表达水平与组织损害的严重程度呈正相关。结论FasL和TIA-1的异常表达在靶细胞凋亡中具有一定作用,其定量检测可能有助于移植肾急性排斥的早期诊断。Objective To study the quantitative method of determination of Fas ligand （FasL） and T-cell intracellular antigen-1 （TIA-1） in renal allografts and to evaluate its clinical significance. Methods The magnitude of intragraft FasL and TIA-1 mRNA was quantified by competitive PCR in 42 samples obtained to diagnose the etiology of graft dysfunction, and the results were expressed as the ratio of picog-rams of target gene to picograms of β-actin, and were compared with the histopathogical diagnosis based on Banff criteria. Results Of all 42 samples, the transcripts were detectable in 11 for FasL and 27 for TIA-1. Intragraft expression of FasL and TIA-1 was significantly ulyregulated in acute rejection group compared with chronic rejection group （P〈0.05） and in patients with no evidence of rejection （P〈 0. 05）. The magnitude of the expression in acute rejection samples showed a correlation with the histologic scores of the samples. At a threshold level, up-regulation of either or both genes demonstrated a sensitivity of 93% and a specificity of 71% for acute rejection. Condusion The abnormal expression strongly indicated that FasL and TIA-1 played a role in the apoptosis of target cells, and the quantitative detection might be valuable for early predictive diagnosis of acute rejection of renal allografts.

关 键 词：肾移植 移植物排斥 FasL/TIA-1 

分 类 号：R699.2[医药卫生—泌尿科学]