S-甲基异硫脲对阿霉素致大鼠心肌组织超氧化物歧化酶、谷胱甘肽过氧化物酶活性改变的影响  

Effect of S-methylisothiourea on activities of superoxide dismutase and glutathione peroxidase in myocardial tissues of rats treated with adriamycin

作　　者：阳冠明[1] 孙胜涛[1] 李树全[1] 林伟雄[1] 叶司原 利基林 林善修[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021 [2]广西肿瘤研究所生化室,广西南宁530021

出　　处：《中国病理生理杂志》2006年第12期2349-2354,共6页Chinese Journal of Pathophysiology

基　　金：广西自然科学基金资助项目(No.0447050)

摘　　要：目的:探讨S-甲基异硫脲(SMT)对阿霉素(ADM)所致大鼠心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性改变的影响。方法:给大鼠腹腔注射ADM(10.0 mg.kg-1)1次,给ADM处理的大鼠静脉注射不同剂量的SMT(每天1次,共3次)进行干预。用分光光度法测定心肌组织丙二醛(MDA)含量、NO2-/NO3-含量、锰超氧化物歧化酶(MnSOD)活性、铜-锌超氧化物歧化酶(Cu-ZnSOD)活性、谷胱甘肽过氧化物酶(GPx)活性、一氧化氮合酶活性;用酶的速率法测定血清肌酸激酶(CK)的同功酶CK-MB活性;用逆转录-聚合酶链反应方法检测心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA、诱导型一氧化氮合酶(iNOS)mR-NA表达。结果:SMT(5.0,10.0,20.0 mg.kg-1)3个干预组心肌组织MDA含量、NO2-/NO3-含量、iNOS活性及血清CK-MB活性明显低于ADM组(P<0.01),而其心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA表达水平及其酶活性明显高于ADM组(P<0.01)。结论:SMT拮抗ADM抑制心肌组织MnSOD mRNA、Cu-ZnSODmRNA、GPx mRNA表达而拮抗ADM抑制这些酶的活性。AIIM ： To study the effect of S - methylisothiourea （SMT） on adriamycin （ADM） - induced changes of superoxide dismutase and alutathione peroxidase activities in myocardial tissue of rats. METHODS ： Rats were treated with ADM by intraperioneal injection （ 10. 0 mg/kg body weight）, and then the ADM - treated rats were intervened by SMT at different dosages by intravenous injection （ once a day for three days）. The contents of malondialdehyde （MDA） and NO2^ -/ NO3^ - , the activities of manganes superoxied dismutase （MnSOD）, copper- zinc superoxide dismutase （Cu - Zn SOD）, glutathione peroxidase （GPx） and inducible nitric oxide synthase （iNOS） were determined by spectrophotome-try in myocardial tissue. The activity of isoenzymic CK - MB of creatine kinase （CK） was determined by enzymic rate method in serum. The expression of MnSOD mRNA, Cu - ZnSOD mRNA, GPx mRNA and iNOS mRNA were detected by reverse transcription -ploymerase chain reaction. RESULTS： The contents of MDA, NO2^-/ NO3^-, and iNOS activity of myocardial tissue, the activity of CK- MB in serum in SMT （5.0, 10. 0, 20. 0 mg/kg） intervention groups were significandy lower than those in ADM - treated group （P 〈 0. 01 ）. However, the activities of MnSOD, Cu - ZnSOD and GPx in myocardial tissue and levels of mRNA expression were significantly higher than those in ADM -treated group （P 〈 0. 01 ）. CONCLUSION ： SMT antagonizes the inhibitory effects of ADM on the expression of MnSOD mRNA, Cu - ZnSOD mRNA and GPx mRNA in myocardial tissue, there by antagonizes the inhibitory effects of ADM on the activities of MnSOD,Cu- ZnSOD and GPx in myocardial tissue.

关 键 词：S-甲基异硫脲 多柔比星 一氧化氮合酶 一氧化氮 超氧化物歧化酶 谷胱甘肽过氧化酶 基 因表达 

分 类 号：R979.1[医药卫生—药品] R977.3[医药卫生—药学]