口蹄疫病毒受体牛源β1亚基基因的分子特征  

Molecular cloning and characteristics of cDNA encoding bovine β1 subunit as FMDV receptor

作　　者：独军政[1] 常惠芸[1] 易华山[1] 丛国正[1] 邵军军[1] 林彤[1] 才学鹏[1] 谢庆阁[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2006年第12期945-950,共6页Chinese Veterinary Science

基　　金：国家重点基础研究发展计划(973)项目(2005CB523201)

摘　　要：从口蹄疫病毒试验感染康复牛肺组织中克隆了β1亚基基因,并对其核苷酸和推导氨基酸序列进行了比较分析。结果显示,牛β1亚基基因的编码区含有2 397个核苷酸,编码798个氨基酸,含有10个潜在的糖基化位点,其中信号肽由20个氨基酸组成,胞外域由708个氨基酸组成,跨膜区由29个氨基酸组成,胞浆域由41个氨基酸组成。与GenBank中的牛β1基因的同源性达99.5%,从起始密码子开始共有12个碱基发生了变化,引起第217、247、268、281、321、691、709位的氨基酸改变,分别由F、S、W、V、H、Y、V变为S、P、R、G、Y、C、G。牛β1基因与猪、猩猩、猫、犬、人、小鼠和鸡的β1基因核苷酸序列同源性分别为93.8%、89.3%、91.6%、90.2%、89.6%、85.4%、75.6%,推导氨基酸序列同源性分别为98.2%、93.7%、97.5%、96.7%、94.2%、93.2%、84.1%。牛与猪β1亚基同源性最高。The bovine integrinβ1 gene was cloned from RNA of the lung tissue of recovered cattle infected experimentally with foot-and-mouth disease virus（FMDV）, and compared with theβ1 genes of other animals available in GenBank at nucleotide and amino acid levels. The complete coding sequence of the bovineβ1 subunit, gene comprised 2 397 bp coding a 798-amino acid-residue protein of ten potential N-linked glycosylation sites, a 20-residue putative signal peptide, a 708 residue ectodomain,a 29-residue transmembrane domain, and a 41-residue cytoplasmic domain. The similarity of nucleotide sequence was 99.5% approximately between the cloned gene and the bovineβ1 gene in Genbank, and 12 nucleotides were changed and 7 amino acids F,S,W,V,H,Y and V were replaced by S,P,R,G,Y,C and G, respectively. The similarity of nucleotide sequence of integrinβ1 gene was 93.8%,89.3%,91.6%,90.2%,89.6%,85.4% and 75.6%, and the amino acid sequence similarity of integrinβ1 was 98.2%,93.7%,97.5%,96.7%,94.2%, 93.2% and 84.1% between cattle and pig, chimpanzee, cat, dog, human, mouse and chicken, respectively. Theβ1 gene between cattle and pig exhibited the highest sequence similarity.

关 键 词：口蹄疫病毒 受体 牛β1亚基基因 分子特征 

分 类 号：S852.659.6[农业科学—基础兽医学]