培养神经元网络对不同间隔电脉冲刺激的响应  

Responses of Cultured Neuronal Network to Electric Pulses Stimuli with Different Intervals

作　　者：陈传平[1] 陈琳[1] 林云生[1] 曾绍群[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《航天医学与医学工程》2006年第6期442-446,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(30370463;30328014)

摘　　要：目的分析培养神经元网络对不同时间间隔电脉冲刺激动作电位发放的影响。方法对培养在多电极阵列(multielectrode arrays,MEA)芯片上的大鼠海马神经元网络加入不同时间间隔的2次电脉冲。结果随刺激间隔时间减小,第2次响应持续时间增加。典型的是,10ms间隔的刺激,第2次响应持续时间有很大增加,并且出现快响应(10ms内)和慢响应(接近或超过100ms)2个阶段。而100ms间隔的刺激,两次响应之间没有明显联系,延时短,锋电位幅值低,个数少,与采用独立单个脉冲刺激实验结果类似。结论培养神经元网络发放受不同电刺激模式的影响。与自发发放比较,不同刺激分别使网络的活动明显得到增强或受到抑制。Objective To analyze the activities of cultured neuronal network in response to electric pulses stimuli with different intervals. Method Pairs of electric pulses with different intervals were added to rat hippocampal neurons cultured on multi-electrode arrays （MEA） substrate. Result The second response duration of the network increased with the decrease of the interval between two pul- ses. It was typical that the second duration after pulses with 10 ms interval increased greatly and it could be divided into two stages, i.e. quick response （within 10 ms） and slow response （near or beyond 100 ms）. However, following pulses with 100 ms interval,there is practically no relation between the two responses; the second response duration was shorter and the number of spikes was fewer and their amplitudes were lower, which is similar to the result of single electric pulse stimulus. Conclusion The activity of cultured neuronal network was different in response to different electric stimulation patterns. Compared with spontaneous firing, the activity of the network is potentiated or depressed respectively after different stimulations.

关 键 词：培养神经元网络 多电极阵列 电刺激 诱导响应 突触修饰 

分 类 号：R338.8[医药卫生—人体生理学]