甘蓝花药培养胚状体诱导形成影响因子研究  

Factors Affecting Embryoid Induction and Formation of Cabbage Anthers in Culture

作　　者：张恩慧[1] 欧承刚[1] 许忠民[1] 程永安[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2006年第11期2372-2377,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家863计划项目(04-145-88);陕西省自然科学基金项目(2004C117)

摘　　要：用甘蓝F1、F2和自交系S33个世代6种基因型材料进行甘蓝花药培养诱导胚状体形成影响因子研究。结果显示:(1)高浓度蔗糖对甘蓝胚状体形成具有显著的诱导作用,6%蔗糖浓度是甘蓝花药培养的最适浓度,其胚状体的诱导率最高达12.2%;(2)材料基因型是影响花药培养的主要因素,F2和F1代材料胚状体诱导效果好,且胚状体诱导率F2代(F2P192和F2P194)18.9%比F1代(F1S17和F1S13)17.1%较高,但差异不显著,自交系S3代材料很难诱导出胚状体;(3)B5培养基比MS培养基更适合甘蓝花药胚状体的诱导培养。结果表明,甘蓝F2代是其花药诱导培养胚状体的最佳基因型材料,B5+2.0 mg/L 2,4-D+2.0 mg/L KT+6%Suc是甘蓝花药诱导培养胚状体的最适培养基。Six genotypes in three generations,cabbage F1 and F2 and inbred S3 were used in anther culture to study the factors affecting embryoid induction and formation of cabbage anthers in culture. It was shown that （1） high sucrose concentration exerted significant inductive effect on ernbryoid formation and the optimum sucrose concentration of cabbage anther culture was 6% and the embryoid induction rate reached as high as 12.2% at this sucrose concentration; （2） The genotypes of the materials were the major factor affecting cabbage anther culture and the materials in cabbage F1 and F2 performed better in embryoid induction and the embryoid induction rate in F2 （F2P192 and F2P194） was 18.9% ,higher but not significantly than the embryoid induction rate of 17.1% in F1 （F1S17 and F1S13） and the inbred materials in S3 were difficult to be induced to produce embryoids; （3） B5 medium was more suitable to induce cabbage anthers to produce embryoids in culture than MS medium. These indicated that cabbage materials in F2 were the best materials for embryoid induction of cabbage anthers in culture and the optimal medium for embryoid induction of cabbage anthers wasB5＋2.0 mg/L2,4-D＋2.0 mg/L KT＋6% sucrose.

关 键 词：甘蓝 花药 组织培养 诱导 胚状体 

分 类 号：Q813.1[生物学—生物工程] S635[农业科学—蔬菜学]