森林脑炎病毒疫苗株的全基因组序列分析  

Sequencing of whole genome of Tick-borne encephalitis vaccine strain

作　　者：宋宗明[1] 赵大鹏[1] 刘双军[1] 王晓宏[1] 赵建[1] 李彤[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《微生物学免疫学进展》2006年第4期7-9,共3页Progress In Microbiology and Immunology

基　　金：国家"十五"重大科技专项(863)(2004AA2Z3630)

摘　　要：实验设计针对森林脑炎病毒的特异性引物,以被森林脑炎疫苗株病毒感染的鼠脑组织中提取的总RNA为模板,用PCR方法分段逆转录合成、扩增序列并测序,应用DNASTAR软件比较分析。结果表明,该病毒疫苗株的全基因组由10782个核苷酸组成,编码3414个氨基酸。森林脑炎疫苗株病毒全基因序列的测定,为研究该病毒疫苗株的生物学特性提供了分子基础。Sequencing of whole genome of Tick-home encephalitis vaccine （ TBEV ） strain was carried out in this study, the total RNA was extracted from the mice infected with TBEV strain, and amplified by PCR based on designed primers. The RT-PCR products of the RNA were purified, sequenced and analyzed by DNASTAR software. The result shows that the whole genome is consisted of 10782 nucleotides encoding 3414 amino acids respectively. It laid a molecular foundation for the investigation of biological characters of TBEV strain.

关 键 词：森林脑炎病毒疫苗株 基因组 序列测定 

分 类 号：R392[医药卫生—免疫学]