HCV的Core-NS3嵌合抗原在大肠杆菌中的表达、纯化及初步应用  

Expression,purification of HCV Core-NS3 chimeric antigen in E.coli and its application

作　　者：彭祥兵[1] 周志军[1] 桑爱军[1] 余健[1] 黄仕和[1] 余模松[1] 

机构地区：[1]武汉生物制品研究所,武汉430060

出　　处：《微生物学免疫学进展》2006年第4期14-18,共5页Progress In Microbiology and Immunology

摘　　要：用已经构建的含有HCV的Core-NS3(C33 c)嵌合基因的表达质粒pGEX TL1-2在大肠杆菌中进行了高效表达,表达产物纯化后通过SDS-PAGE、W estern-b lot、ELISA等一系列鉴定试验进行了分析,结果表明,该嵌合抗原具有高度特异性和良好的抗原活性,为研制诊断用HCV抗原奠定基础。The constructed expression plasmid pGEX TL1-2 which contains chimeric gene of HCV Core-NS3 （ C33c ） was highly expressed. The purified recombinant protein was analyzed by SDS-PAGE, Western-blot and indirect ELISA, respectively. The prepared chimeric antigen showed high specificity and good antigenicity, and it laid a foundation for studying and developing of HCV diagnostic antigens.

关 键 词：HCV Core-NS3 嵌合抗原 

分 类 号：R392[医药卫生—免疫学]