肌氨酸氧化酶法检测血清中肌酐含量的方法学评价  

To Evaluate the Enzymatic Method for Assaying Creatinine in Serum

作　　者：张宏斌[1] 邓敬仪[1] 

机构地区：[1]中山大学附属第三医院,广东广州510630

出　　处：《实用医技杂志》2006年第22期3927-3928,共2页Journal of Practical Medical Techniques

摘　　要：目的评价肌氨酸氧化酶法(简称酶法)检测血清中肌酐含量的准确度、特异性和精确度。方法在设制好参数的前提下,取样本,于日立7170全自动生化分析仪上,进行重复、回收、干扰等一系列试验,同时用苦味酸法(Jaffe法)进行相同实验,对比其结果。结果酶法测定肌酐的批内变异系数(CV)为0.67%,批间CV为0.83%,线性范围0μmol/L^8840μmol/L,胆红素(≥165.5μmol/L)和溶血(Hb<1g/L)干扰接近零,回收率为101.7%。准确度、精确度和特异性均较Jaffe法高。结论酶法抗黄疸干扰力强,操作简单、准确、重复性好,可作为常规测定方法。Objective To evaluate the precise,accurate and specific of enzymatic method for assaying creatinine in serum.Methods Both Enzymatic method and Jaffe＇s method were used in HITACH17170A automatic analyzr.After the pare meter of it was build them compared those results.Results The method affords a simple.Rapid and sensitive assay with good precision,extended linearity much better in clinical needs.Conclusion The method is easily automated and is suiable for routine work in clinical laboratories.

关 键 词：肌氨酸 酶学测定 苦味酸法 血清 

分 类 号：R446.11[医药卫生—诊断学]