海藻酸钠明胶协同固定化的研究  

Study on the Immobilized Sodium Alginate-Gelatin

作　　者：王爱玲[1] 杨江科[1] 尹利[1] 闫云君[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《生物技术》2006年第6期69-72,共4页Biotechnology

基　　金：国家高技术研究发展计划(863计划)项目("生物柴油关键技术及应用研究";2003AA214061);湖北省科技攻关项目资助

摘　　要：目的:研究不同因素对固定化微胶囊的影响以及不同物质向微胶囊扩散的规律。方法:采用海藻酸钠与明胶协同固定化制备微胶囊,考察了海藻酸钠、明胶浓度等因子对微胶囊直径与机械强度的影响,以及牛血清蛋白与葡萄糖向微胶囊扩散的状况,并利用非稳态传递模型计算了这两种物质在微胶囊中的有效扩散系数。结果:随着海藻酸钠质量浓度的升高,微胶囊的直径与机械强度逐渐增大。制备的最优条件是CaCl2浓度为10%,滴定速度为180滴/min,最优浸泡时间为30min。在此条件下,葡萄糖与牛血清蛋白的有效扩散系数分别为4.63×10-5cm2/min、1.01×10-7cm2/min。结论:海藻酸钠明胶协同固定化制备的微胶囊作为一种生物载体,非常适合细胞或酶的固定化。Objeetive：Effeets of immobilization conditions and diffusion of solution into the mieroeapsule were studied. Methods： Sodium alginate - gelatin miemeapsule was prepared. The effects of eoneentrations of sodium alginate（SA） and gelatin（GT）on the diameter and compression intensity were studied, diffusion coefficients from the solution into the microeapsule of substrate, glucose and Bovine serum albumin Fraction （BSA） were also investigated according to the established mathematical model. Results： With the increasing of sodium alginate eoneentration, the diameter and eompression intensity became bigger, and the optimum operation eonditions were： the eoneentration of ealeium chloridea 10%, drop rate 180 droplets/min, dipped in ealeium chloridea 30min, respeetively. The effective diffusion parameters of glueose and BSA were 4.63 × 10^-5cm^2/min and 1.01 ×10^-7cm^2/min, respectively. Conclusion： Sodium alginate- gelatin mierocapsule is a promising immobilization carrier and best for cell and enzyme immobilized.

关 键 词：海藻酸钠 明胶 压缩强度 有效扩散参数 

分 类 号：Q814.2[生物学—生物工程]